Using Prenatal Blood Samples to Evaluate COVID-19 Rapid Serologic Tests Specificity

Maternal and Child Health Journal
Jackeline AlgerPierre Buekens

Abstract

Background cross-reactivity with other coronaviruses may reduce the specificity of COVID-19 rapid serologic tests. The vast majority of women attend prenatal care, which is a unique source of population-based blood samples appropriate for validation studies. We used stored 2018 serum samples from an existing pregnancy cohort study to evaluate the specificity of COVID-19 serologic rapid diagnostic tests. We randomly selected 120 stored serum samples from pregnant women enrolled in a cohort in 2018 in Tegucigalpa, Honduras, at least 1 year before the COVID-19 pandemic. We used stored serum to evaluate four lateral flow rapid diagnostic tests, following manufacturers' instructions. Pictures were taken for all tests and read by two blinded trained evaluators. We evaluated 120, 80, 90, and 90 samples, respectively. Specificity for both IgM and IgG was 100% for the first two tests (95% confidence intervals [CI] 97.0-100 and 95.5-100, respectively). The third test had a specificity of 98.9% (95% CI 94.0-100) for IgM and 94.4% (95% CI 87.5-98.2) for IgG. The fourth test had a specificity of 88.9% (95% CI 80.5-94.5) for IgM and 100% (95% CI 96.0-100) for IgG. COVID-19 serologic rapid tests are of variable specificity. Blood specimens fr...Continue Reading

References

Oct 15, 2010·Clinical and Vaccine Immunology : CVI·Geoffrey J GorseTheresa Z O'Connor
Oct 15, 2013·Reproductive Health·Pierre BuekensConcepcion Zuniga
Mar 29, 2014·Virus Research·Benjamin MeyerMarcel A Müller
Jul 18, 2016·Reproductive Health·Pierre BuekensUNKNOWN ZIPH Working Group
Aug 2, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Diep Ngoc Thi NguyenAnnette Fox
Dec 7, 2017·The American Journal of Tropical Medicine and Hygiene·Pierre Buekens For The Congenital Chagas Working Group
Apr 25, 2020·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Rodolfo CastroHugo Perazzo
Apr 26, 2020·The Lancet Global Health·Pierre BuekensGiselle Tomasso
May 7, 2020·JAMA : the Journal of the American Medical Association·Nandini SethuramanAkihide Ryo
Jun 5, 2020·Science Translational Medicine·Ralph WeisslederMikael J Pittet

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Citations

Aug 14, 2021·JMIR Public Health and Surveillance·Breno Bernardes-SouzaWendel Coura-Vital

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Methods Mentioned

BETA
IECS

Software Mentioned

Plataforma
OpenEpi
REDCap

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