Using reduced graphene oxide-Ca:CdSe nanocomposite to enhance photoelectrochemical activity of gold nanoparticles functionalized tungsten oxide for highly sensitive prostate specific antigen detection

Biosensors & Bioelectronics
Xueping WangQin Wei

Abstract

An ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor was constructed for the detection of prostate specific antigen (PSA). In this work, Au-nanoparticle-loaded tungsten oxide (WO3-Au) hybrid composites was applied as PEC sensing platform, while Ca ions doped CdSe equipped on the conducting framework of reduced graphene oxide (rGO-Ca:CdSe) nanocomposites were employed as the signal amplification probe. As for WO3-Au, massive Au nanoparticles were formed on the surface of WO3without any additional reducing agent, providing a novel nanocarriers for anchoring plenty of the primary antibodies due to the large specific surface area and good biocompatibility by chemical bonding between Au nanoparticles and -NH2of antibodies. Besides, the incorporation of the rGO and the doping of Ca ions could improve the conductivity and hinder the recombination of electron-hole pairs of CdSe nanoparticles effectively, thereby enhancing the photocurrent conversion efficiency. Based on the sandwich immunoreaction, the primary antibody was immobilized onto WO3-Au substrate, after the formed rGO-Ca:CdSe labels were captured onto the electrode surface via the specific antibody-antigen interaction, the photocurrent intensity could be fu...Continue Reading

Citations

May 4, 2018·Expert Review of Molecular Diagnostics·Shuang Yin, Zhanfang Ma
Apr 25, 2018·Chemistry : a European Journal·Yang ZangHuan Pang
Oct 10, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhejian LiChunsheng Zhou
Oct 31, 2020·Research : a Science Partner Journal·Ruitao LiuTianhong Cui
Oct 20, 2018·ACS Applied Materials & Interfaces·Yueyuan LiQin Wei
Dec 12, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Germano Duarte PortoPaulo Cesar Lock Silveira

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