Using reversed-phase liquid chromatography to monitor the sizes of Au/Pt core/shell nanoparticles

Journal of Chromatography. a
Fu-Ken Liu, Yu-Cheng Chang

Abstract

This paper describes the use of reversed-phase liquid chromatography (RPLC) to rapidly characterize Au/Pt core/shell nanoparticles (NPs) produced through seed-assisted synthesis. We monitored the sizes of Au/Pt core/shell NPs by using a porous silica-based RPLC column (pore size: ca. 100 nm) and 30 mM sodium dodecyl sulfate in deionized water as the mobile phase; the plot of the retention time with respect to the logarithm of the size of the Au NPs was linear (R(2)=0.997) for diameters falling in the range from 5.3 to 40.1 nm; from five consecutive runs, the relative standard deviations of these retention times were less than 0.4%. We used the optimal separation conditions of the RPLC system to study the effects that the rate of addition of the reducing agent and the volumes of the seed, shell precursor metal ion, and reducing agent solutions had on the sizes of the Au/Pt core/shell NPs. A good correlation existed between the sizes of the Au/Pt core/shell NPs determined through RPLC and those determined using transmission electron microscopy. RPLC appears to be a useful technique for monitoring the sizes of NPs and nanomaterials in general.

Citations

Feb 15, 2011·Analytical and Bioanalytical Chemistry·Petr S FedotovBoris Ya Spivakov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.