Using RNAi to investigate orthologous homeotic gene function during development of distantly related insects

Evolution & Development
S J BrownJ W Mahaffey

Abstract

Gene product distribution is often used to infer developmental similarities and differences in animals with evolutionarily diverse body plans. However, to address commonalties of developmental mechanisms, what is really needed is a method to assess and compare gene function in divergent organisms. This requires mutations eliminating gene function. Such mutations are often difficult to obtain, even in organisms amenable to genetic analysis. To address this issue we have investigated the use of double-stranded RNA interference to phenocopy null mutations. We show that RNA interference can be used to phenocopy mutations of the Deformed orthologues in Drosophila and Tribolium. We discuss the possible use of this technique for comparisons of developmental mechanisms in organisms with differing ontogenies.

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J BrownR E Denell
Oct 1, 1993·Journal of the Neurological Sciences·A S WierzbickiP T Lascelles
Nov 1, 1996·Genetics·A MochizukiY Iwasa
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·G PanganibanS B Carroll
Aug 1, 1997·Current Opinion in Genetics & Development·E Robey
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L Misquitta, B M Paterson
Mar 7, 1991·Nature·J J StuartR E Denell

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Citations

Jan 3, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J D Evans, D E Wheeler
May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Denell, T Shippy
Sep 15, 2004·Development Genes and Evolution·Yoshinori Tomoyasu, Robin E Denell
Apr 9, 2008·Development Genes and Evolution·Reinhard SchröderRebekka Lutz
Nov 26, 2008·Development Genes and Evolution·Teresa D ShippyRobin E Denell
Feb 18, 2011·Development Genes and Evolution·Yasuhiko KatoHajime Watanabe
Aug 15, 2012·Development Genes and Evolution·Johannes Benno SchinkoGregor Bucher
Nov 8, 2002·Insect Molecular Biology·M BeyeS W Omholt
Nov 9, 2006·Nucleic Acids Research·Liangjiang WangSusan J Brown
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Neema AgrawalSunil K Mukherjee
Sep 5, 2002·Annual Review of Entomology·David G Heckel
May 21, 2010·BMC Developmental Biology·Johannes B SchinkoGregor Bucher
Jan 18, 2013·BMC Genomics·Peter KitzmannGregor Bucher
Nov 2, 2012·EvoDevo·Joshua F Coulcher, Maximilian J Telford
Sep 9, 1999·Nursing Management·D Barnett
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S BrownR Denell
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·M StauberU Schmidt-Ott
Jan 16, 2013·Annual Review of Entomology·Alison Heffer, Leslie Pick
Mar 21, 2001·Evolution & Development·C L Hughes, T C Kaufman
Dec 21, 2002·Evolution & Development·Cynthia L Hughes, Thomas C Kaufman
Mar 19, 2014·FEBS Letters·Daniel K Hasegawa, Matthew W Turnbull
May 29, 2012·Insect Biochemistry and Molecular Biology·Jennie S Garbutt, Stuart E Reynolds
Aug 31, 2010·Arthropod Structure & Development·Nico PosnienGregor Bucher
Nov 4, 2008·Biochimica Et Biophysica Acta·Miriam I RosenbergClaude Desplan
Nov 21, 2007·Molecular Phylogenetics and Evolution·David R Angelini, Elizabeth L Jockusch
Jul 27, 2007·Evolution & Development·Armin P MoczekDebra J Rose
Nov 23, 2013·Evolution & Development·Alison HefferLeslie Pick
Dec 3, 2010·Archives of Insect Biochemistry and Physiology·H XiangY P Huang
Apr 9, 2016·Development Genes and Evolution·Nikolaus Dieter Bernhard KoniszewskiGregor Bucher
Jun 30, 2006·Mechanisms of Development·Hilde WohlfromGregor Bucher
Dec 23, 1999·Current Biology : CB·G Gibson
Sep 18, 2013·Journal of Insect Physiology·Jeffrey G ScottAngela E Douglas
Dec 18, 2007·Developmental Biology·Kristen A Panfilio

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