Using scanning electron microscopy (SEM) to study morphology and morphometry of the isolated haptoral sclerites of three distinct diplozoid species

PloS One
Q M Dos SantosAnnemariè Avenant-Oldewage

Abstract

Diplozoidae infects the gills of cyprinid fishes in Africa, Europe, and Asia. Traditionally the hardened internal structures, crucial for identification of diplozoid species, are studied using light microscopy. Recently, the sclerotised haptoral structures of an African diplozoid, Paradiplozoon vaalense, were successfully isolated and visualised using scanning electron microscopy (SEM). In this paper, the haptoral sclerites of three diplozoid species are compared using SEM for the first time. Paradiplozoon ichthyoxanthon and Paradiplozoon vaalense occur on Labeobarbus and Labeo species, respectively, in the Vaal River system, South Africa, while Diplozoon paradoxum is widely-distributed in Europe and Asia, infecting several host species. Diplozoon paradoxum is a well-studied species, as well as being the type species of the family and ideal for inclusion in an exploratory study for comparative purposes. SEM study of D. paradoxum and P. ichthyoxanthon provided valuable information regarding surface morphology of the attachment structures hitherto not observed. Elaborate morphometric study of the haptoral hooks were incorporated, adding 12 point-to-point measurements to current morphometric characteristics. The results were analy...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 27, 2001·Parasitology·I MatejusováC O Cunningham
Aug 26, 2014·The Journal of Parasitology·Quinton Marco Dos Santos, Annemarie Avenant-Oldewage
Apr 2, 2015·Parasitology Research·Wouter FannesGiuseppe Paladini

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Datasets Mentioned

BETA
AF369759
AJ563372
KP326299
HF566124
HG423142

Methods Mentioned

BETA
light microscopy
scanning electron microscopy
PCA

Software Mentioned

BLAST
Geneious R6

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