Using sequential immunoprecipitation and mass spectrometry to identify methylation of NF-κB

Methods in Molecular Biology
Tao Lu, George R Stark

Abstract

Posttranslational modifications have long been known to play an essential role in the regulation of NF-κB activity. In the past few years, in addition to more traditional modifications such as phosphorylation, the p65 subunit of NF-κB has been found to be methylated at multiple sites. Here, we describe procedures for using immunoprecipitation and mass spectrometry to identify the methylation sites of p65.

Citations

Jan 13, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Suzanne AcklooSusanne Müller
Sep 5, 2015·Cancer Research·Tao Lu, George R Stark
Aug 23, 2018·Molecular Cancer Research : MCR·Yang Yang-HartwichGil Mor

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