Using sindbis viral vectors for specific detection and suppression of advanced ovarian cancer in animal models

Cancer Research
Jen-Chieh TsengD Meruelo

Abstract

We studied the therapeutic value of Sindbis vectors for advanced metastatic ovarian cancer by using two highly reproducible and clinically accurate mouse models: a SCID xenograft model, established by i.p. inoculation of human ES-2 ovarian cancer cells, and a syngenic C57BL/6 model, established by i.p. inoculation of mouse MOSEC ovarian cancer cells. We demonstrate through imaging, histologic, and molecular data that Sindbis vectors systemically and specifically infect/detect and kill metastasized tumors in the peritoneal cavity, leading to significant suppression of the carcinomatosis in both animal models. Use of two different bioluminescent genetic markers for the IVIS Imaging System permitted demonstration, for the first time, of an excellent correlation between vector delivery and metastatic locations in vivo. Sindbis vector infection and growth suppression of murine MOSEC tumor cells indicate that Sindbis tumor specificity is not attributable to a species difference between human tumor and mouse normal cells. Sindbis virus is known to infect mammalian cells using the Mr 67,000 laminin receptor. Immunohistochemical staining of tumor cells indicates that laminin receptor is elevated in tumor versus normal cells. Down-regula...Continue Reading

References

Jan 1, 1984·Breast Cancer Research and Treatment·S H BarskyL A Liotta
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V P TerranovaL A Liotta
Aug 19, 1993·International Journal of Cancer. Journal International Du Cancer·J M LiebmanH K Kleinman
Mar 3, 1993·Journal of the National Cancer Institute·S MartignoneM I Colnaghi
May 15, 1998·Journal of Cellular Biochemistry·S ButòS Ménard
Aug 8, 1998·Journal of Virology·A P Byrnes, D E Griffin
Oct 30, 1998·Japanese Journal of Clinical Oncology·G de ManzoniC Cordiano
Apr 3, 1999·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R RiegerS Weiss
Apr 7, 2000·Carcinogenesis·K F RobyP F Terranova
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Bhaumik, S S Gambhir
Dec 5, 2002·Journal of the National Cancer Institute·Jen-Chieh TsengDaniel Meruelo
Sep 1, 1955·The American Journal of Tropical Medicine and Hygiene·R M TAYLORT E FROTHINGHAM
Dec 3, 2003·Nature Biotechnology·Jen-Chieh TsengDaniel Meruelo

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Citations

Aug 1, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lars StelterSteven M Larson
Nov 13, 2008·Expert Reviews in Molecular Medicine·Gregory J AtkinsBrian J Sheahan
Aug 11, 2012·Molecular Pharmaceutics·Fan ChengSuchetana Mukhopadhyay
Sep 8, 2012·Cancer Gene Therapy·R SuzmeA Pellicer
Sep 3, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jonathan ScheimanDaniel Meruelo
Jan 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yu-Qian ZhangChien-Fu Hung
Nov 10, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pong-Yu HuangLih-Hwa Hwang
Sep 13, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tomer GranotDaniel Meruelo
Aug 2, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Juan R Rodriguez-MadozCristian Smerdou
Nov 22, 2005·Oncogene·J Michael MathisDavid T Curiel
Apr 13, 2006·Rejuvenation Research·Alicia HurtadoDaniel Meruelo
Oct 19, 2007·Cancer Research·Chih-Long ChangChien-Fu Hung
Aug 4, 2010·Journal of Drug Targeting·Elena AppelDavid Mirelman
Jan 21, 2016·Immunotherapy·Ruben Hernandez-AlcocebaCristian Smerdou
Apr 6, 2012·Virus Research·Christine PampenoDaniel Meruelo
Aug 10, 2010·Virus Research·Jose I QuetglasCristian Smerdou
Mar 27, 2007·Cancer Letters·Markus J V Vähä-KoskelaAri E Hinkkanen
Aug 28, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Masafumi ToyoshimaNobuo Yaegashi
Mar 28, 2008·Immunological Reviews·Chien-Fu HungRichard Roden
Oct 21, 2010·Luminescence : the Journal of Biological and Chemical Luminescence·Luke G GutweinStephen R Grobmyer
Aug 20, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alicia HurtadoDaniel Meruelo
Oct 19, 2005·Gene Therapy·E PiverJ-C Pagès
Mar 1, 2005·Gene Therapy·K W Peng, E Galanis
May 30, 2013·International Journal of Molecular Medicine·Anna HyvärinenRiikka Pellinen

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