Using specific antisera to neutralize ACTH in sturgeon: a method for manipulating the interrenal response during stress

General and Comparative Endocrinology
S E LankfordJ J Cech

Abstract

Interrenal function and the magnitude of the stress response were assessed in green sturgeon (Acipenser medirostris) passively immunized with antisera directed against adrenocorticotropic hormone (ACTH). The nucleotide sequence encoding ACTH was determined using reverse transcriptase polymerase chain reaction (RT-PCR). We identified two isoforms of ACTH that differ at a single site (position 26) in the 39 AA peptide. Both forms of green sturgeon ACTH (gsACTH1-39) display 100% homology with both sequences of white sturgeon ACTH (wsACTH1-39). The N-terminal portion of gsACTH also shares absolute identity with the comparable portion of human ACTH (hACTH). However, we identified considerable sequence divergence in the C-terminal domain between gsACTH and hACTH. Species-specific anti-ACTH sera were generated by vaccinating sheep against the C-terminal portion of gsACTH (gsACTH26-39). The peptide was covalently linked to a carrier protein (keyhole-limpet-hemocyanin [KLH]) to further enhance its immunogenicity. The anti-gsACTH sera recognized gsACTH1-39 and the immunogenic peptide (gsACTH26-39), but did not interact with hACTH1-39. To assess the impact of the antisera, fish were passively immunized with anti-gsACTH26-39 sera or anti-K...Continue Reading

References

May 23, 1985·Journal of Immunological Methods·A WhiteJ G Ratcliffe
Jun 1, 1986·General and Comparative Endocrinology·J P SumpterT J Benfey
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S G ReidS F Perry
Jan 22, 2000·General and Comparative Endocrinology·N W Pankhurst, G Van Der Kraak
Jul 11, 2002·The Journal of Clinical Endocrinology and Metabolism·Zongjuan FangSerdar E Bulun
Jun 19, 2003·General and Comparative Endocrinology·Mathilakath M VijayanRamesh Sathiyaa
Feb 26, 2004·General and Comparative Endocrinology·Jessica L CostaUte Hochgeschwender
Jun 17, 1950·British Medical Journal·H SELYE
May 14, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Juriaan R MetzGert Flik
Jul 1, 2002·Integrative and Comparative Biology·Neil GreenbergCliff H Summers
Jul 1, 2002·Integrative and Comparative Biology·Firdaus S Dhabhar

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Citations

Apr 4, 2009·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·L S KrayushkinaA Ogozhalek
May 31, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Samaneh PoursaeidGlen Van Der Kraak

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