Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Alexander W PetersonAnne L Plant

Abstract

Spatially resolved details of the interactions of cells with a fibronectin modified surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free technique that is based on the spatial measurement of interfacial refractive index. SPRI is sensitive to short range interactions between cells and their substratum. The high contrast in SPR signal between cell edges and substratum facilitates identification of cell edges and segmentation of cell areas. With this novel technique, we demonstrate visualization of cell-substratum interactions, and how cell-substratum interactions change over time as cells spread, migrate, and undergo membrane ruffling.

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Feb 28, 2009·BMC Cell Biology·Alexander W PetersonAnne L Plant
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Citations

Aug 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Wei WangNongjian Tao
Mar 13, 2014·Sensors·Yuhki YanaseMichihiro Hide
Dec 3, 2014·BMC Cell Biology·Alexander W PetersonAnne L Plant
Aug 13, 2013·Biophysical Journal·Marc P RaphaelJeff M Byers
Dec 10, 2013·Sensors and Actuators. B, Chemical·Natalya ZaytsevaYe Fang
Sep 27, 2015·Biosensors & Bioelectronics·Jong-Ryul ChoiKyujung Kim
Jun 17, 2015·Annual Review of Analytical Chemistry·Joachim Wegener
Jan 22, 2013·Biophysical Journal·Marc P RaphaelJeff M Byers
Mar 10, 2016·Clinical and Experimental Immunology·Z SzittnerG Vidarsson
Oct 27, 2016·The Review of Scientific Instruments·Alexander W PetersonJohn T Elliott
Jun 17, 2020·Optics Letters·Hossein HassaniAndreas Offenhäusser
Mar 28, 2018·Analytical Chemistry·Xiaoying Wang, Feimeng Zhou

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