Using Transcriptomes as Mutant Phenotypes Reveals Functional Regions of a Mediator Subunit in Caenorhabditis elegans

Genetics
David Angeles-Albores, Paul W Sternberg

Abstract

Although transcriptomes have recently been used as phenotypes with which to perform epistasis analyses, they are not yet used to study intragenic function/structure relationships. We developed a theoretical framework to study allelic series using transcriptomic phenotypes. As a proof-of-concept, we apply our methods to an allelic series of dpy-22, a highly pleiotropic Caenorhabditis elegans gene orthologous to the human gene MED12, which encodes a subunit of the Mediator complex. Our methods identify functional units within dpy-22 that modulate Mediator activity upon various genetic programs, including the Wnt and Ras modules.

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Citations

Aug 28, 2020·ELife·Fabrice BesnardMarie-Anne Félix
Oct 13, 2020·RNA Biology·Fabian BraukmannEric Alexander Miska
Jul 22, 2021·PLoS Genetics·Amanda L FryE Jane Albert Hubbard

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