Metagenomic survey unravel diversity of biogas microbiome with potential to enhance its' productivity in Kenya

BioRxiv : the Preprint Server for Biology
Samuel Mwangangi MuturiR. Pelle


The obstacle to optimal utilization of biogas technology is poor understanding of biogas' microbiome diversities over a wide geographical coverage. We performed random shotgun sequencing on the twelve treatments to four blocks, within eastern and central regions of Kenya. We obtained 42 million paired-end reads that were annotated against sixteen reference databases using two ENVO ontologies, prior to beta-diversity studies. We identified 37 phyla, 65 classes and 132 orders. Bacterial richness dominated and composed 28 phyla, 42 classes and 92 orders, conveying substrate's versatility in the treatments. Though fungi and Archaea composed 5 phyla, the fungi were richer; suggesting importance of hydrolysis and fermentation in biogas production. High beta-diversity within the taxa was largely linked to communities' metabolic capabilities. Clostridiales and Bacteroidales, the most prevalent guilds, metabolized organic macromolecules. Cytophagales, Alteromonadales, Flavobacteriales, Fusobacteriales, Deferribacterales, Elusimicrobiales, Chlamydiales, Synergistales to mention but few, also catabolized macromolecules into smaller substrates to conserve energy. Furthermore, delta-Proteobacteria, Gloeobacteria and Clostridia affiliates sy...Continue Reading

Related Concepts

Metabolic Process, Cellular
Genome-Wide Association Study
Genetic Association Studies
Body Mass Index Procedure
DNA Binding Domain
FTO gene

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.