Using ZIF-8 as stationary phase for capillary electrophoresis separation of proteins

Talanta
Zhongmin GengHailin Cong

Abstract

Recently, the separation of proteins has received much attention, although many techniques require expensive instrumentation and trained analysts. In this work, a low-cost, effective, and environmental friendship capillary electrophoresis (CE) for proteins separation was first time introduced. The ZIF-8 with outstanding properties of large surface area, and accessible tunnels and cages were coated the inner surface of silica capillary as a separation media by electrostatic interaction. The fast baseline separation of Lys, CC, BSA and RNase A can be obtained within 10 min using the ZIF-8 nanocrystals coated capillary column under the optimum separation conditions. Meanwhile, this system showed good reproducibility and stability. Using L-glutamic acid as the selector ligand, the D- and L-phenylalanine were successfully separated by the ZIF-8 nanocrystals coated capillary column. Furthermore, the method was also applied to separate egg white proteins, and three main proteins were separated in a single run.

Citations

Jan 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sofia C VardaliDimitrios A Giannakoudakis
May 8, 2019·Electrophoresis·Shimeles Addisu KitteGuobao Xu
Sep 18, 2020·Advances in Colloid and Interface Science·Shixiang LiuHailin Cong
Mar 24, 2021·Analytical and Bioanalytical Chemistry·Ziting Gao, Wenwan Zhong
Apr 10, 2021·Water Environment Research : a Research Publication of the Water Environment Federation·Jiun-Hau OuChih-Ming Kao
Nov 9, 2019·Analytical Chemistry·Courtney J KristoffLisa A Holland

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