USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair

Frontiers in Cell and Developmental Biology
Yixuan ZhaoQingfeng Li

Abstract

Re-epithelialization is a fundamental process in wound healing that involves various cytokines and cells during cutaneous barrier reconstruction. Ubiquitin-specific peptidase 15 (USP15), an important member of the deubiquitinating enzymes (DUBs), removes ubiquitin chains from target proteins and maintains protein stability. However, the dynamic role of USP15 in epithelialization remains unclear. We aimed to investigate the regulatory function of USP15 in re-epithelialization. An excisional wound splinting model was established to evaluate the re-epithelialization rate in Usp15 knockout (KO) mice. Coimmunoprecipitation (Co-IP) and mass spectrum analyses were performed to identify USP15-interacting proteins. RNA-sequencing was performed for transcriptome analysis in keratinocytes and uploaded into NODE database (http://www.biosino.org/node, accession numbers: OEP000770 and OEP000763). First, a significant delay in epithelialization was observed in the Usp15 KO mice. Moreover, inhibition of cell migration and proliferation was observed in the USP15-silenced keratinocytes (HaCaTs). Moreover, we revealed for the first time that USP15 could interact with eukaryotic initiation factor 4A-1 (EIF4A1), thereby promoting translational effi...Continue Reading

References

Apr 2, 2002·The Journal of Surgical Research·Karl W SchwarzScott A Dulchavsky
May 2, 2002·The Journal of Investigative Dermatology·Stacy MazzalupoPierre A Coulombe
Feb 17, 2010·The Journal of Cell Biology·Annika WolfMechthild Hatzfeld
Sep 11, 2010·Nature·Byung-Hoon LeeDaniel Finley
Sep 29, 2011·Nature Cell Biology·Masafumi InuiStefano Piccolo
Mar 14, 2013·Development·Barbara A Schmidt, Valerie Horsley
Jun 4, 2013·Molecular Cell·Nicole F VilleneuveDonna D Zhang
May 24, 2014·Human Molecular Genetics·Tom CornelissenWim Vandenberghe
Jan 24, 2015·Cell Death and Differentiation·M Stoneley, A E Willis
Feb 2, 2016·The Journal of Investigative Dermatology·Joanna JackówClaus-Werner Franzke
Nov 24, 2016·Cancer Research·Cailin E JoyceCarl D Novina
Mar 11, 2017·Pharmacology & Therapeutics·Mingjing HeYong Wan
Jun 13, 2017·Experimental Dermatology·Nathalie DeshayesMaryline Paris
Mar 30, 2018·Nature Communications·Achuth PadmanabhanJoAnne S Richards
May 29, 2018·Trends in Cell Biology·Emanuel Rognoni, Fiona M Watt
May 31, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tara L PetersJonathan H Schatz
Feb 13, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xuhui MaYongzhang Luo
Oct 1, 2019·The Journal of Investigative Dermatology·Ana TellecheaAristidis Veves
May 12, 2020·PLoS Genetics·Fernando Mejías-NavarroPablo Huertas

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Datasets Mentioned

BETA
OEP000763

Methods Mentioned

BETA
biopsy
electrophoresis
RNA Assay
coimmunoprecipitation
flow cytometry
transfection
RNA-seq
interaction
immunoprecipitation assay
ubiquitination

Software Mentioned

Gene Set Enrichment Analysis ( GSEA )
GraphPad Prism
TopHat
GraphPad
Cufflinks

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