USP27-mediated Cyclin E stabilization drives cell cycle progression and hepatocellular tumorigenesis.

Oncogene
Ling DongZhenghong Lin

Abstract

Overexpression of Cyclin E has been seen in many types of cancers. However, the underlying mechanism remains enigmatic. Herein, we identified ubiquitin-specific peptidase 27 (USP27) as a Cyclin E interactor. We found that USP27 promoted Cyclin E stability by negatively regulating its ubiquitination. In addition, suppression of USP27 expression resulted in the inhibition of the growth, migration, and invasion of hepatocellular carcinoma. Furthermore, we detected a positive correlation between USP27 and Cyclin E expression in hepatocellular carcinoma tissues. Finally, we found that USP27 expression is inhibited by 5-fluorouracil (5-FU) treatment and USP27 depletion sensitizes Hep3B cells to 5-FU-induced apoptosis. USP27-mediated Cyclin E stabilization is involved in tumorigenesis, suggesting that targeting USP27 may represent a new therapeutic strategy to treat cancers with aberrant overexpression of Cyclin E protein.

References

May 1, 1995·Molecular and Cellular Biology·M OhtsuboM Pagano
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Keyomarsi, A B Pardee
Sep 1, 1996·Virchows Archiv : an International Journal of Pathology·W YasuiE Tahara
Sep 28, 1999·Nature·C H SpruckS I Reed
Sep 29, 1999·Genes & Development·J D SingerJ M Roberts
Apr 25, 2000·Molecular and Cellular Biology·E Santoni-RugiuJ Lukas
Jun 28, 2001·Leukemia & Lymphoma·M Erlanson, G Landberg
Nov 7, 2002·Current Biology : CB·Alex C MinellaBruce E Clurman
Nov 15, 2002·The New England Journal of Medicine·Khandan KeyomarsiSarah S Bacus
Jul 8, 2003·The Journal of Pathology·Peter SchramlGuido Sauter
Jun 12, 2004·European Journal of Endocrinology·Frédérique TissierChristine Gicquel
Apr 20, 2005·Oncogene·Harry C Hwang, Bruce E Clurman
Apr 21, 2005·Seminars in Cell & Developmental Biology·Irma Sánchez, Brian David Dynlacht
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Joanna Bloom, Frederick R Cross
Jan 17, 2009·Chemical Reviews·Katherine WickliffeMichael Rape
Dec 22, 2011·Cell Cycle·Ka Tat SiuAlex C Minella
Sep 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Po Hien EarStephen W Michnick
Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Satu LuhtalaJorma Isola
Jan 1, 2015·Cell Discovery·Zhenghong LinDeyu Fang
Dec 17, 2016·Cell·Matthew P SwafferPaul Nurse

❮ Previous
Next ❯

Citations

Dec 31, 2019·Cellular and Molecular Life Sciences : CMLS·Jiyeon Seo, Mikyoung Park
Sep 23, 2018·Cells·Esperanza Hernández-CarraleroRaimundo Freire
Aug 27, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Ji ChengWenyi Wei
Jun 3, 2021·International Journal of Molecular Sciences·Tingting Zou, Zhenghong Lin
Jun 3, 2021·International Journal of Molecular Sciences·Arun Pandian ChandrasekaranSuresh Ramakrishna
Jan 13, 2022·Cellular and Molecular Life Sciences : CMLS·Tingting ZouZhenghong Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
deubiquitination
immunoprecipitation
ubiquitination
PCR
flow cytometry
xenograft
light microscopy

Software Mentioned

Image J

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.