USP7 Regulates Cytokinesis through FBXO38 and KIF20B

Scientific Reports
Anna GeorgesLori Frappier

Abstract

The ubiquitin specific protease 7 (USP7 or HAUSP) is known to regulate a variety of cellular processes by binding and deubiquitylating specific target proteins. To gain a more comprehensive understanding of its interactions and functions, we used affinity purification coupled to mass spectrometry to profile USP7 interactions. This revealed a novel interaction with FBXO38, a poorly characterized F-box protein. We showed that USP7 stabilizes FBXO38 dependent on its catalytic activity by protecting FBXO38 from proteasomal degradation. We used a BioID approach to profile the protein interactions (and putative functions) of FBXO38, revealing an interaction with KIF20B, a Kinesin-6 protein required for efficient cytokinesis. FBXO38 was shown to function independently from an SCF complex to stabilize KIF20B. Consequently, depletion of either FBXO38 or USP7 led to dramatic decreases in KIF20B levels and KIF20B at the midbody, which were manifested in cytokinetic defects. Furthermore, cytokinetic defects associated with USP7 silencing were rescued by restoring FBXO38 or KIF20B. The results indicate a novel mechanism of regulating cytokinesis through USP7 and FBXO38.

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Citations

Jul 5, 2019·Expert Review of Proteomics·Benoît BégantonJérôme Solassol
Jul 26, 2019·Journal of Virology·Umama Z SiddiqiLori Frappier
Dec 7, 2020·Journal of Molecular Biology·Nicholas W AshtonRoger Woodgate
Nov 18, 2020·International Journal of Molecular Sciences·Manon BrunetMarlène Dufresne
Oct 30, 2020·International Journal of Molecular Sciences·Jordan BlondelleStephan Lange
Apr 14, 2021·Nucleic Acids Research·Dongxue SuLin Shan

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Datasets Mentioned

BETA
MSV000082444

Methods Mentioned

BETA
affinity
affinity purification
immunoprecipitation
transfection
fluorescence microscopy
PCR
transfections

Software Mentioned

! Tandem
Mascot
Proteowizard
SAINT Express
Leica Application Suite X ( LAS X
iProphet

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