USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET

Reproduction : the Official Journal of the Society for the Study of Fertility
Giovanna Berruti, Chiara Paiardi

Abstract

The acrosome is a peculiar vacuole that at fertilization undergoes the acrosome reaction (AR), an event unique in the sperm life. Contents released promote sperm penetration through oocyte's investments; membranous components are involved in sperm-egg interaction/fusion. Therefore, both constituents play a role in fertilization. The biogenesis of this vacuole, however, has not been clarified yet; recently, it has been proposed as a novel lysosome-related organelle (LRO). Our research focuses on the involvement of the endosomal pathway in acrosomogenesis starting from the early phases. The trafficking sorted by USP8/UBPy, an endosomal regulator recently described as a compelling candidate for male fertility gene, was investigated in comparison to that of SP56, a marker of the biosynthetic pathway. Mouse spermatids were double/triple immunolabeled and examined by confocal microscopy. The contribution of the vesicular traffic assisted by the cortical microtubule array was also evaluated in nocodazole-treated spermatids. USP8/UBPy-sorted cargo contributes early to acrosomogenesis and its trafficking is microtubule mediated. It was identified, through co-immunoprecipitation/co-immunolocalization assays, that the membrane receptor ME...Continue Reading

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Citations

Jan 18, 2016·Seminars in Cell & Developmental Biology·Giovanna Berruti
Mar 29, 2019·Science China. Life Sciences·Zhu HuangQinghua Shi
Dec 18, 2019·Biochemical Society Transactions·Almut Dufner, Klaus-Peter Knobeloch
Jun 21, 2019·Cellular and Molecular Life Sciences : CMLS·Romain Da CostaLaurence Duplomb
Dec 10, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ling ZhangZhibing Zhang
Oct 18, 2019·Frontiers in Cell and Developmental Biology·Muhammad Babar KhawarWei Li
May 26, 2018·Histochemistry and Cell Biology·Giovanna BerrutiEnzo Martegani
Apr 3, 2020·American Journal of Physiology. Cell Physiology·Wei QuZhibing Zhang
Apr 23, 2020·Human Reproduction Update·Christiane PleugerMoira K O'Bryan
Jan 2, 2021·Journal of Personalized Medicine·Miriam Cerván-MartínF David Carmona
Oct 24, 2020·Reproductive Biology·Ayesha YousafXiaohua Jiang
Dec 7, 2021·Microscopy Research and Technique·Maider BizkarguenagaEdurne Alonso

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