Uterine epithelial cells specifically induce interferon-stimulated genes in response to polyinosinic-polycytidylic acid independently of estradiol.

PloS One
Mickey V PatelCharles R Wira

Abstract

Interferon β (IFNβ) is an antiviral cytokine secreted in response to pathogenic exposure that creates a restrictive intracellular environment through the action of downstream interferon-stimulated genes (ISG). The objective of this study was to examine the expression of IFNβ and ISG in both human uterine epithelial cells (UEC) and the ECC-1 uterine epithelial cell line and determine if expression changes with TLR stimulation and hormone exposure. Stimulation of primary uterine epithelial cells and ECC-1 cells with the TLR3 agonist poly (I:C) induced the mRNA expression of IFNβ, MxA, OAS2 and PKR. Other TLR agonists including imiquimod and CpG had no effect on either IFNβ or ISG expression. In contrast to ECC-1 cell responses which were slower, maximal IFNβ upregulation in UEC occurred 3 hours post-stimulation and preceded the ISG response which peaked approximately 12 hours after poly (I:C) exposure. Unexpectedly, estradiol, either alone or prior to treatment with poly (I:C), had no effect on IFNβ or ISG expression. Blockade of the IFN receptor abrogated the upregulation of MxA, OAS2 and PKR. Furthermore, neutralizing antibodies against IFNβ partially inhibited the upregulation of all three ISG. Estradiol, directly and in the p...Continue Reading

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Citations

Apr 25, 2013·PloS One·Marta Rodriguez-GarciaCharles R Wira
Mar 26, 2014·American Journal of Reproductive Immunology : AJRI·Charles R WiraJohn V Fahey
May 20, 2014·American Journal of Obstetrics and Gynecology·Sharon L AchillesSharon L Hillier
Mar 7, 2015·Nature Reviews. Immunology·Charles R WiraMickey V Patel
Aug 5, 2017·American Journal of Reproductive Immunology : AJRI·Jonathan Alexander PasternakHeather L Wilson
Oct 9, 2018·American Journal of Reproductive Immunology : AJRI·Mickey V PatelCharles R Wira
Dec 13, 2018·Clinical and Experimental Reproductive Medicine·Zahra ZandiehReza Aflatoonian
Nov 18, 2019·Seminars in Immunology·Zoe R C MarksPaul J Hertzog
Mar 16, 2021·Current HIV/AIDS Reports·Marta Rodriguez-GarciaMimi Ghosh
May 6, 2021·Aging Cell·Marta Rodriguez-GarciaCharles R Wira

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Methods Mentioned

BETA
hysterectomies
enzyme-linked immunosorbent assay
Assay
PCR
ELISA

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