Uteroglobin messenger ribonucleic acid: localization in rabbit uterus and lung by in situ hybridization

Endocrinology
M WarembourgE Milgrom

Abstract

The messenger RNA (mRNA) coding for uteroglobin has been localized in the rabbit uterus and lung by in situ hybridization. Tissue sections fixed in ethanol-acetic acid were hybridized to the cloned complementary DNA probe labeled with tritium. The hybridization sites were detected by radioautography. Control experiments using [3H]pBR322 DNA demonstrated the specificity of the observed labeling. In the lung, uteroglobin mRNA, present in small concentrations, could be clearly visualized only after background was decreased by incubation of sections with S1 nuclease. In pregnant rabbit uterine horns, uteroglobin mRNA, visualized by silver grains, was found in the endometrial epithelium. The concentration was greater in the cells of glandular epithelium than in the cells of surface epithelium. Specific and intense labeling was spread through the cytoplasm. Practically all epithelial cells contained uteroglobin mRNA. Hybridization was very weak in the uterine epithelial cells of the nonpregnant rabbit. In the lung, a high degree of labeling occurred on the ciliated and bronchiolar cells of the epithelium of bronchi and bronchioles whereas the goblet cells remained unlabeled. Certain cells lining alveolar ducts and alveoli in the pulm...Continue Reading

Citations

Jan 1, 1991·Anatomy and Embryology·D R DannhornC Kirchner
Jul 1, 1991·American Journal of Respiratory Cell and Molecular Biology·K Mihal, N Riedel
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Feb 24, 2001·Annals of the New York Academy of Sciences·H M Beier
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