Utilisation of Ambient Laser Desorption Ionisation Mass Spectrometry (ALDI-MS) Improves Lipid-Based Microbial Species Level Identification

Scientific Reports
Simon CameronZoltan Takats

Abstract

The accurate and timely identification of the causative organism of infection is important in ensuring the optimum treatment regimen is prescribed for a patient. Rapid evaporative ionisation mass spectrometry (REIMS), using electrical diathermy for the thermal disruption of a sample, has been shown to provide fast and accurate identification of microorganisms directly from culture. However, this method requires contact to be made between the REIMS probe and microbial biomass; resulting in the necessity to clean or replace the probes between analyses. Here, optimisation and utilisation of ambient laser desorption ionisation (ALDI) for improved speciation accuracy and analytical throughput is shown. Optimisation was completed on 15 isolates of Escherichia coli, showing 5 W in pulsatile mode produced the highest signal-to-noise ratio. These parameters were used in the analysis of 150 clinical isolates from ten microbial species, resulting in a speciation accuracy of 99.4% - higher than all previously reported REIMS modalities. Comparison of spectral data showed high levels of similarity between previously published electrical diathermy REIMS data. ALDI does not require contact to be made with the sample during analysis, meaning an...Continue Reading

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Citations

Nov 25, 2019·Rapid Communications in Mass Spectrometry : RCM·Joscelyn SarsbyRobert J Beynon
Feb 13, 2020·MSystems·Arunima BhattacharjeeChristopher R Anderton
Mar 2, 2021·Journal of the American Society for Mass Spectrometry·Jana HavlikovaHelen J Cooper
Oct 18, 2019·ACS Synthetic Biology·Glen-Oliver F GowersZoltan Takáts
Dec 10, 2021·Journal of the American Society for Mass Spectrometry·Sylvia P NautaRon M A Heeren

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Methods Mentioned

BETA
PCA

Software Mentioned

MetaboAnalyst
Offline Model Building ( OMB )
learn
ALDI
plotly
js
REIMS
scikit
OMB
Cytoscape

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