Utility of 5-Cyanotryptophan Fluorescence as a Sensitive Probe of Protein Hydration

The Journal of Physical Chemistry. B
Beatrice N MarkiewiczFeng Gai

Abstract

Tryptophan (Trp) fluorescence has been widely used to interrogate the structure, dynamics, and function of proteins. In particular, it provides a convenient and site-specific means to probe a protein's hydration status and dynamics. Herein, we show that a tryptophan analogue, 5-cyanotryptophan (TrpCN), can also be used for this purpose, but with the benefit of enhanced sensitivity to hydration. This conclusion is reached based on measurements of the static and time-resolved fluorescence properties of 5-cyanoindole, TrpCN, and TrpCN-containing peptides in different solvents, which indicate that upon dehydration the fluorescence quantum yield (QY) and lifetime (τF) of TrpCN undergo a much greater change in comparison to those of Trp. For example, in H2O the QY of TrpCN is less than 0.01, which increases to 0.11 in 1,4-dioxane. Consistently, the fluorescence decay kinetics of TrpCN in H2O are dominated by a 0.4 ns component, whereas in 1,4-dioxane the kinetics are dominated by a 6.0 ns component. The versatile utility of TrpCN as a sensitive fluorescence reporter is further demonstrated in three applications, where we used it (1) to probe the solvent property of a binary mixture consisting of dimethyl sulfoxide and H2O, (2) to mon...Continue Reading

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Citations

Aug 5, 2016·Physical Chemistry Chemical Physics : PCCP·Andres HenaoLuis Carlos Pardo
Aug 2, 2016·The Journal of Physical Chemistry. B·G X NieJ P Huang
Sep 7, 2016·Current Opinion in Chemical Biology·Pallavi M Gosavi, Ivan V Korendovych
Oct 21, 2016·Physical Chemistry Chemical Physics : PCCP·Beatrice N MarkiewiczFeng Gai
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Bei DingFeng Gai
Jul 19, 2018·Physical Chemistry Chemical Physics : PCCP·L J G W van WilderenJ Bredenbeck
Sep 6, 2018·Physical Chemistry Chemical Physics : PCCP·Michael SchneiderMichael Schmitt
Apr 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min YouWenkai Zhang
Jun 11, 2019·Physical Chemistry Chemical Physics : PCCP·Ismail A AhmedFeng Gai
Jun 22, 2019·Physical Chemistry Chemical Physics : PCCP·Marie-Luise HebestreitMichael Schmitt
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mary Rose HilaireFeng Gai
Dec 12, 2017·Chemical Physics Letters·Mary Rose HilaireFeng Gai
May 13, 2017·International Journal of Biological Macromolecules·Nida Zaidi, Rizwan Hasan Khan
May 6, 2021·Physical Chemistry Chemical Physics : PCCP·Marie-Luise HebestreitMichael Schmitt
May 25, 2021·The Journal of Physical Chemistry. B·Arusha AcharyyaFeng Gai
Aug 3, 2019·The Journal of Physical Chemistry. B·Salsabil Abou-Hatab, Spiridoula Matsika
May 4, 2017·Journal of the American Chemical Society·Josef M MaierKen D Shimizu
May 17, 2018·The Journal of Physical Chemistry Letters·Bei DingFeng Gai
Feb 1, 2017·The Journal of Physical Chemistry. a·Josefin WilkeMichael Schmitt
Jul 11, 2018·Organic Letters·Kellan T Passow, Daniel A Harki
Nov 2, 2021·Biotechnology and Bioengineering·Madison M MannBryan W Berger

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