Utility of anion and cation combinations for phasing of protein structures.

Journal of Structural and Functional Genomics
Ashwani SharmaAmit Sharma

Abstract

We report the use of anionic (I(-)), cationic (Ba(2+), Cd(2+)) and ionic mixtures (I(-) plus Ba(2+)) for derivatizing liver fatty acid binding protein (LFABP) crystals. Use of cationic and anionic salts in phasing experiments revealed distinct non-overlapping sites for these ions, suggesting exclusive binding regions on LFABP. Interestingly, cations of identical charge and valency (like Ba(2+) and Cd(2+)) bound to distinct pockets on the protein surface. Furthermore, a mixture of salts containing both I(-) and Ba(2+) was very useful in phasing experiments as these oppositely charged ions bound to different regions of LFABP. Our data therefore suggest that cationic and anionic salt mixtures like BaCl(2) with NH(4)I or salts like CdI, BaI where each ion has a significant anomalous signal for a given X-ray wavelength may be valuable reagents for phasing during structure determination.

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Citations

Jan 15, 2014·Acta Crystallographica. Section D, Biological Crystallography·Salla RuskamoPetri Kursula
Mar 24, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yao XuShan Mou
Jul 31, 2013·Journal of Mass Spectrometry : JMS·C SantambrogioH Molinari
Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sándor GondaMárta M-Hamvas
Aug 14, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Yi-Yun ChengPing-Chiang Lyu

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