PMID: 9537278Apr 16, 1998Paper

Utility of antibodies against a 22 kD molecule of Dirofilaria immitis in the diagnosis of human pulmonary dirofilariasis

Tropical Medicine & International Health : TM & IH
L PereraA Muro

Abstract

To assess the characteristics of an ELISA test for the diagnosis of human pulmonary dirofilariasis, we studied the sera of 24 subjects with other helmintoses and of 37 patients suffering from non-parasitic focal lung diseases, comparing them with negative and positive sera. ELISA and Western blot with complete somatic antigen and ELISA with protein Di22 (specifically recognized in cases of lung dirofilariasis) were performed. With ELISA SA the false positive rate was 25% in cases with other parasitoses and 30% in cases with focal lung diseases. ELISA Di22 decreases this positivity levels. Only 2 cases with visceral larva migrans (8.3%) and a case with lung nodules metastatic from renal adenocarcinoma (2.7%) were positive. ELISA Di22 therefore greatly decreases the false positive rate of ELISA SA.

References

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Citations

Aug 26, 1999·Parasitology Today·A MuroF Simón
Feb 12, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cristina Carranza-RodriguezJ L Pérez-Arellano
Jul 6, 2012·Clinical Microbiology Reviews·Fernando SimónJose Alberto Montoya-Alonso
Dec 21, 2006·Emerging Infectious Diseases·Javier PardoJosé-Luis Pérez-Arellano
Feb 13, 2001·Tropical Medicine & International Health : TM & IH·C VieiraF Simón
Apr 11, 2001·Chest·B C BielawskiE Joseph
Oct 18, 2011·Experimental Parasitology·Ji Seoung ParkSoon Cheol Ahn
Oct 4, 2005·Veterinary Parasitology·F SimónJ R Martín-Pacho
Jul 25, 2014·Medicine·Moncef Belhassen-GarcíaMiguel Cordero-Sánchez
Aug 28, 2020·Frontiers in Veterinary Science·María Victoria Esteban-MendozaRodrigo Morchón
Nov 13, 2004·Journal of Computer Assisted Tomography·Yasuji OshiroKazuhiko Yamada
Feb 25, 2017·PLoS Neglected Tropical Diseases·Cristina Carranza-RodríguezJosé-Luis Pérez-Arellano
Jan 12, 2016·Paediatrics and International Child Health·Moncef Belhassen-GarcíaMiguel Cordero-Sánchez
Nov 3, 2020·Frontiers in Veterinary Science·Sara SavićRodrigo Morchón

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