PMID: 9542927May 23, 1998Paper

Utility of major leukocyte subpopulations for monitoring secondary cytomegalovirus infections in renal-allograft recipients by PCR

Journal of Clinical Microbiology
P SchäferR Laufs

Abstract

The feasibility of the major peripheral blood leukocyte (PBL) subsets for use in qualitative and quantitative PCR to monitor secondary cytomegalovirus (CMV) infection and ganciclovir therapy was assessed with 188 blood samples derived from 40 CMV immunoglobulin G-positive renal-allograft recipients. In pp65 antigen-positive patients all leukocyte fractions, but only 79.5% of plasma preparations, were PCR positive. In pp65 antigen-negative samples from patients after antiviral treatment only 7.3% of polymorphonuclear cell (PMNL) samples, but 81.8% of peripheral blood mononuclear cells (PBMC), and 10.9% of plasma samples remained PCR positive. Similarly, in patients with latent infections only 5.0% of PMNL, but 51.7% of PBMC preparations, and 8.0% of plasma samples were PCR positive. Regarding patients with active CMV infection, CMV DNA copy numbers in PMNL correlated significantly with pp65 antigen-positive cell counts before and after onset of ganciclovir therapy. Significant differences in CMV DNA copy numbers in PMNL and plasma were observed (i) between patients with symptomatic infection and those with asymptomatic infection and (ii) between patients with active infection and those with latent infection. In contrast, PBMC ha...Continue Reading

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Citations

Jun 5, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W van der Bij, R Speich
Sep 12, 2000·Current Gastroenterology Reports·R W Goodgame
Aug 23, 2005·Memórias do Instituto Oswaldo Cruz·Meltem Yalinay CirakSukru Sindel
Oct 5, 2013·Clinical Microbiology Reviews·Raymund R Razonable, Randall T Hayden
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·L Rasmussen
Oct 7, 2005·Journal of Clinical Microbiology·Wafa HabbalBarbara Christine Gärtner
Nov 1, 2002·Journal of Clinical Microbiology·René BoomMenno D De Jong
Jun 29, 2002·Journal of Clinical Microbiology·Jason L Sanchez, Gregory A Storch
Oct 6, 2001·Gastroenterology Clinics of North America·R W Goodgame

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