Abstract
We developed a method for the identification of Aspergillus fumigatus fungal isolates by using random amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) cloning and the TaqMan LS50B fluorogenic detection system (Perkin-Elmer Corp., Applied Biosystems, Foster City, Calif.). DNA from seven clinically important Aspergillus species was screened by RAPD-PCR to identify section- or species-specific amplicons. With the OPZ19 RAPD primer a 1,264-bp product was amplified from all A. fumigatus strains initially examined but not from other species. A partial DNA sequence of this product was used to design a specific primer pair, which generated a single 864-bp fragment with DNA from 90 of 100 A. fumigatus isolates when a "touchdown" (65-->55 degrees C) annealing protocol was used. The TaqMan system, a fluorogenic assay which uses the 5'-->3' endonuclease activity of Taq DNA polymerase, detected this 864-bp product with DNA from 89 of these 90 A. fumigatus strains; 1 DNA sample generated an indeterminate result. With DNA from three morphologically typical A. fumigatus isolates, six white ("albino") A. fumigatus isolates, and five of six Neosartorya species (non-A. fumigatus members of the section Fumigati), the 864-bp product was amplified di...Continue Reading
References
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Nov 11, 1989·Nucleic Acids Research·W Rychlik, R E Rhoads
Oct 1, 1995·Applied and Environmental Microbiology·H A BasslerC A Batt
Jun 1, 1995·PCR Methods and Applications·K J LivakK Deetz
Jul 1, 1995·Journal of Clinical Microbiology·M E BrandtR W Pinner
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·T G MitchellW G Merz
Apr 1, 1995·Applied and Environmental Microbiology·H GirardinJ P Latgé
Jul 1, 1994·Journal of Clinical Microbiology·W J MelchersJ F Meis
Aug 1, 1993·Molecular Ecology·R FaniM Bazzicalupo
Nov 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·V T Andriole
Apr 1, 1993·Journal of Clinical Microbiology·H G NiestersW G Quint
Nov 1, 1995·Journal of Clinical Microbiology·G S SandhuG D Roberts
May 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J R Perfect, W A Schell
Jan 1, 1996·Mycoses·R KappeM Maiwald
Dec 1, 1996·Journal of Clinical Microbiology·T MorrisM Gallagher
Apr 1, 1997·Journal of Clinical Microbiology·D C SwanJ P Icenogle
Citations
Jul 20, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D BuchheidtR Hehlmann
Mar 12, 2002·British Journal of Haematology·Dieter BuchheidtRüdiger Hehlmann
Sep 17, 2002·Medical Mycology·Sharon C A ChenWieland Meyer
Nov 23, 2018·Genome Génome / Conseil National De Recherches Canada·Wieland MeyerUNKNOWN ISHAM barcoding of pathogenic fungi working group
Sep 25, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Theresa HahnPhilip L McCarthy
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·J C BowmanC M Douglas
Oct 24, 2008·Phytopathology·R D FrederickD A Knorr
Aug 22, 2007·Medical Mycology·S Arunmozhi BalajeeMary E Brandt
Jul 5, 2002·Clinical Microbiology Reviews·Siew Fah Yeo, Brian Wong
Nov 10, 2009·Future Microbiology·Anna LauTania Sorrell
Aug 31, 2000·Applied and Environmental Microbiology·A D JourdanI V Wesley
Apr 9, 2001·Molecular and Cellular Probes·P Cruz-PerezL D Stetzenbach
Jan 7, 2005·Journal of Clinical Microbiology·Sergey V BalashovDavid S Perlin
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Jul 6, 2000·Journal of Clinical Microbiology·G E KillgoreF C Tenover
May 15, 1999·Journal of Clinical Microbiology·A PahlA Gessner
May 31, 2002·Journal of Clinical Microbiology·Juergen LoefflerHermann Einsele
Mar 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I M Mackay
Feb 3, 2000·Journal of Clinical Microbiology·J LoefflerH Einsele
Apr 23, 2011·BMC Microbiology·Rita SerranoRicardo Araujo