Abstract
Currently available Interferon-γ release assay (IGRA) cannot reliably differentiate active TB (ATB) from non-active TB (non-ATB). A study was performed to evaluate the value of Mycobacterium tuberculosis (MTB) specific Th1 cell immune responses which test IFN-γ and IL-2 simultaneous for differentiating ATB from non-ATB. Forty-nine newly diagnosed inpatients with ATB (26 pulmonary TB and 23 extrapulmonary TB) were enrolled as the ATB group. Forty-five volunteers with latent tuberculosis infection (LTBI) and twenty with evidence of previous TB were enrolled during the same period as the non-ATB group. Clinical examination and MTB specific Th1 cell immune responses were performed for all participants. After being stimulated with ESAT-6 and CFP-10, the median frequencies of single IL-2-, single IFN-γ-, and dual IFN-γ/IL-2-secreting T-cells were all higher in the ATB group than in the non-ATB group (20(8-45) vs. 7(3-13), P<0.001;131(44-308) vs. 10(6-27), P<0.001;25(9-74) vs. 7(3-23), P = 0.001, respectively). Evaluation of the diagnostic performance of detecting single IFN-γ-secreting T cells for pulmonary TB employed a cutoff value of 35 iSFCs/250,000 PBMC. The sensitivity, specificity, positive predictive value (PPV), negative pre...Continue Reading
References
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerry A MillingtonAjit Lalvani
Jun 1, 2007·The European Respiratory Journal·J-P JanssensT Rochat
Jun 15, 2007·Chest·Ajit Lalvani
Jul 3, 2008·Annals of Internal Medicine·Madhukar PaiDick Menzies
Nov 21, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C B E CheeY T Wang
Jun 25, 2009·BMC Infectious Diseases·Valeria SargentiniRoberto Nisini
Nov 6, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R BiselliR Nisini
Dec 24, 2010·PloS One·Rosalyn CaseyAjit Lalvani
Oct 26, 2011·The Journal of Infectious Diseases·John Z MetcalfeMadhukar Pai
Oct 27, 2012·Tuberculosis and Respiratory Diseases·Yoon-Sung ParkSang-Sook Shin
Jan 15, 2014·PloS One·Lifan ZhangXiaoqing Liu
Jan 31, 2014·Infection·P BittelF Suter-Riniker
Aug 30, 2014·Respiration; International Review of Thoracic Diseases·Paulin N EssoneChristoph Lange
Nov 8, 2014·Clinical Rheumatology·Qiaoling RuanWenhong Zhang
Feb 15, 2015·The Lancet Infectious Diseases·Lei GaoUNKNOWN LATENTTB-NSTM study team
Mar 19, 2015·PloS One·Dumitru ChesovBarbara Kalsdorf
May 23, 2015·Journal of Clinical Microbiology·Ashutosh N AggarwalDigambar Behera
Nov 20, 2016·Medicine·Xiaoqing LiuZhiyong Liang
Citations
Mar 6, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Chiara Della BellaLuisa Galli
Nov 30, 2018·Bulletin of Experimental Biology and Medicine·V A SerebryakovaT E Kononova
Nov 22, 2021·Chinese Medical Journal·Lifan ZhangXiaoqing Liu