Utilization of amino acids by Trypanosoma brucei in culture: L-threonine as a precursor for acetate.

Parasitology
G A CrossD J Linstead

Abstract

The amino acid compositions of several culture media have been analysed and compared. The utilization and excretion of amino acids and other metabolites have been followed during growth of Trypanosoma brucei S42 in a defined medium. All of the added L-threonine was metabolized by the cells, even when it was present at elevated concentrations. Glucose was consumed throughout the growth cycle: glutamine was consumed more rapidly than glutamic acid, which was itself used at about the same rate as proline. Threonine was cleaved to form glycine and acetate, both of which accumulated in the medium. Alanine and succinate were excreted together with a small amount of pyruvate, but these three products accounted for less than half of the glucose used. CO2 production from glucose was not measured, but insignificant amounts of CO2 were produced from threonine. Tetraethylthiuram disulphide blocked the cleavage of threonine and was a potent inhibitor of trypanosome growth.

References

Apr 1, 1972·Comparative Biochemistry and Physiology. A, Comparative Physiology·G C Southworth, C P Read
Jul 18, 1973·Nature: New Biology·V Kilgour, D G Godfrey
Nov 1, 1972·The Journal of protozoology·D A Evans, R C Brown
Dec 1, 1973·Parasitology·G A Cross, J C Manning
Jan 1, 1973·Transactions of the Royal Society of Tropical Medicine and Hygiene·H P Voorheis
May 1, 1974·The Journal of protozoology·M D Ruff, C P Read
Sep 1, 1969·Comparative Biochemistry and Physiology·D G Dusanic
Jun 1, 1958·Journal of General Microbiology·G W KIDDER, B N DUTTA
Mar 1, 1951·The Journal of Pharmacy and Pharmacology·W D GRAHAM
Jan 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·J F MORGANR C PARKER

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Citations

Jan 1, 1980·The International Journal of Biochemistry·V Kilgour
Jan 1, 1977·Transactions of the Royal Society of Tropical Medicine and Hygiene·R F Steiger, S R Meshnick
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·J R Baker, L F Selden
Feb 1, 1981·Molecular and Biochemical Parasitology·E L JarrollS A Morse
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J Cazzulo
Mar 23, 2005·Trends in Parasitology·Sébastien BesteiroFrédéric Bringaud
Nov 1, 2001·Trends in Parasitology·K S PaulP T Englund
May 1, 1978·Antimicrobial Agents and Chemotherapy·J Williamson, T J Scott-Finnigan
Nov 14, 2003·Kinetoplastid Biology and Disease·Véronique HannaertPaul AM Michels
May 19, 2009·BMC Systems Biology·Seth B RobertsGregory A Buck
Oct 7, 2006·Parasitology Research·Appolinaire DjikengClotilde Carlow
Sep 13, 2014·BMC Microbiology·Carla V De Paula LimaFabricio K Marchini
May 15, 2012·International Journal for Parasitology·Jurgen R HaanstraBarbara M Bakker
Sep 13, 2011·International Journal for Parasitology·Muriel MazetPaul A M Michels
Sep 15, 2009·Trends in Parasitology·Aloysius G M Tielens, Jaap J van Hellemond
Feb 12, 2008·Molecular and Biochemical Parasitology·Jeremy W ChambersJames C Morris
Apr 17, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cristina Nowicki, Juan J Cazzulo
May 10, 2006·Molecular and Biochemical Parasitology·Frédéric BringaudVirginie Coustou
Oct 27, 2011·Molecular and Biochemical Parasitology·Darren J CreekMichael P Barrett
May 7, 2015·PLoS Pathogens·Juan P de MacêdoPeter Bütikofer
Mar 25, 2016·The Journal of Eukaryotic Microbiology·Fred R OpperdoesJulius Lukeš
May 20, 2018·The FEBS Journal·Natasha SienkiewiczAlan H Fairlamb
Sep 24, 2016·PLoS Pathogens·Víctor M Castillo-AcostaDolores González-Pacanowska
Mar 17, 2007·Nature Reviews. Microbiology·Soo Hee LeePaul T Englund
Sep 1, 1987·Journal of Veterinary Pharmacology and Therapeutics·V B Braide

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