Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof

Journal of Food Science and Technology
K ThirunavukarasuN R Kamini

Abstract

Fish meal has been used as an additional nitrogen source and fish oil as inducer for the growth and production of lipase from Cryptococcus sp. MTCC 5455. A response surface design illustrated that the optimum factors influencing lipase production were fish meal, 1.5 %, w/v, Na2HPO4, 0.2 %, w/v, yeast extract, 0.25 %, w/v and sardine oil, 2.0 %, w/v with an activity of 71.23 U/mL at 96 h and 25 °C, which was 48.39 % higher than the conventional one-factor-at-a-time method. The crude concentrated enzyme hydrolyzed polyurethane (PUR) efficiently and hydrolysis was 94 % at 30 °C and 96 h. The products, diethylene glycol and adipic acid were quantified by HPLC and scanning electron microscopic studies of the degraded polymer showed significant increase in size of the holes from 24 to 72 h of incubation. Hydrolysis of PUR within 96 h makes the lipase novel for disposal of PUR and provides an innovative solution to the problems created by plastic wastes.

References

Jul 1, 1997·Infection and Immunity·L C ChenA Casadevall
Apr 27, 2000·Journal of Bacteriology·G PignèdeJ M Nicaud
Jul 13, 2007·Applied Biochemistry and Biotechnology·R GautamE K Yanful
Mar 10, 2015·Journal of Food Science and Technology·Vrinda RamakrishnanBhaskar Narayan

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Citations

Apr 28, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Adegoke Isiaka Adetunji, Ademola Olufolahan Olaniran
Jul 10, 2021·Frontiers in Bioengineering and Biotechnology·Efstratios NikolaivitsJasmina Nikodinovic-Runic
Nov 7, 2019·Biotechnology Advances·Audrey MagninLuc Avérous

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