Jan 1, 1982

Utilization of ketone bodies and glucose by established neural cell lines

Journal of Neuroscience Research
L M RoederJ T Tildon

Abstract

The rates of utilization of [3-14C]-acetoacetate, [3-14C]-3-hydroxybutyrate, and [6-14C]-glucose were measured in four established cell lines from neuroblastoma of rat (B103) and mouse (N4TG1) and from rat astrocytoma (RGC6) and mouse oligodendroglia (G2620). The rates of incorporation of acetoacetate into lipid were 3-5 times higher than glucose in all cell lines. The incorporation of 3-hydroxybutyrate was similar to that of glucose. Thin-layer chromatography of the total lipid extracts showed the same relative rates of use of these substrates for synthesis of various phospholipids and neutral lipids. The rates of incorporation into neutral lipids and phosphatidylcholine were essentially linear for 12 hr; however, that into phosphatidylethanolamine was markedly higher in the second 6 hr interval than in the first. In all cases, the greatest percentage of label (35-50%) appeared in the phosphatidylcholine fraction. The distribution of label from each of the three substrates among the various lipids was similar in the glial cells, but there were marked differences in distribution of the two ketone bodies in the neuroblastoma lines. These cells also synthesized lipids that migrated to the same area on the chromatogram as choleste...Continue Reading

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Mentioned in this Paper

Lipogenesis
Central Neuroblastoma
Hydroxybutyric Acids
Mixed Oligoastrocytoma
Neurons
Brain
Ketone Bodies Measurement, Quantitative
Glucose, (beta-D)-Isomer
Neuroglia
Phosphatidylethanolamine

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