PMID: 8972698Dec 4, 1996Paper

Utilization of monensin for detection of microdomains in cholesterol containing membrane

Biochimica Et Biophysica Acta
S Bransburg-ZabaryM Gutman

Abstract

The effect of cholesterol on the monensin mediated proton-cation exchange reaction was measured in the time-resolved domain. The experimental system consisted of a black lipid membrane equilibrated with monensin (Nachliel, E., Finkelstein, Y. and Gutman, M. (1996) Biochim. Biophys. Acta 1285, 131-145). The membrane separated two compartments containing electrolyte solutions and pyranine (8-hydroxypyrene 1,3,6-trisulfonate) was added on to one side of the membrane. A short laser pulse was used to cause a brief transient acidification of the pyranine-containing solution and the resulting electric signal, derived from proton-cation exchange, was measured in the microsecond time domain. Incorporation of cholesterol had a clear effect on the electric transients as measured with Na+ or K+ as transportable cations. The measured transients were subjected to rigorous analysis based on numeric integration of coupled, non-linear, differential rate equations which correspond with the perturbed multi-equilibria state between all reactants present in the system. The various kinetic parameters of the reaction and their dependence on the cholesterol content had been determined. On the basis of these observations we can draw the following concl...Continue Reading

References

May 22, 1995·Chemistry and Physics of Lipids·N BorochovD Bach
Mar 13, 1996·Biochimica Et Biophysica Acta·C H SpinkM Breed
Dec 4, 1996·Biochimica Et Biophysica Acta·E NachlielM Gutman

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Citations

May 29, 2003·Biochimie·E G Berger, J Rohrer
Jan 1, 1997·Annual Review of Physical Chemistry·M Gutman, E Nachliel
Dec 15, 2010·Cell Structure and Function·Masaya OkuHiderou Yoshida
Feb 15, 2005·Biochemical and Biophysical Research Communications·Gabriela NegroiuStefana M Petrescu
Dec 4, 1996·Biochimica Et Biophysica Acta·E NachlielM Gutman
Feb 6, 2019·Scientific Reports·Marion VannesteMichael D Henry

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