Utilization of n-alkanes by a newly isolated strain of Acinetobacter venetianus: the role of two AlkB-type alkane hydroxylases

Applied Microbiology and Biotechnology
Mimmi Throne-HolstSergey B Zotchev

Abstract

A bacterial strain capable of utilizing n-alkanes with chain lengths ranging from decane (C10H22) to tetracontane (C40H82) as a sole carbon source was isolated using a system for screening microorganisms able to grow on paraffin (mixed long-chain n-alkanes). The isolate, identified according to its 16S rRNA sequence as Acinetobacter venetianus, was designated A. venetianus 6A2. Two DNA fragments encoding parts of AlkB-type alkane hydroxylase homologues, designated alkMa and alkMb, were polymerase chain reaction-amplified from the genome of A. venetianus 6A2. To study the roles of these two alkM paralogues in n-alkane utilization in A. venetianus 6A2, we constructed alkMa, alkMb, and alkMa/alkMb disruption mutants. Studies on the growth patterns of the disruption mutants using n-alkanes with different chain lengths as sole carbon source demonstrated central roles for the alkMa and alkMb genes in utilization of C10 to C18 n-alkanes. Comparative analysis of these patterns also suggested different substrate preferences for AlkMa and AlkMb in n-alkane utilization. Because both single and double mutants were able to grow on n-alkanes with chain lengths of C20 and longer, we concluded that yet another enzyme(s) for the utilization of ...Continue Reading

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Citations

Jan 5, 2014·The Journal of Microbiology·Fei HuaYi Cun Zhao
Aug 4, 2007·Applied Microbiology and Biotechnology·Alexander WentzelMimmi Throne-Holst
Jun 11, 2011·Applied Microbiology and Biotechnology·Sandrine PaisseMarisol Goñi-Urriza
Mar 4, 2011·The Journal of Microbiology·Yoon-Suk KangWoojun Park
Mar 27, 2013·Research in Microbiology·Marco FondiRenato Fani
Apr 3, 2007·Applied and Environmental Microbiology·Mimmi Throne-HolstSergey B Zotchev
Dec 25, 2010·Applied and Environmental Microbiology·Luca Lo PiccoloPaola Quatrini
Nov 17, 2010·PloS One·Olivia U MasonStephen J Giovannoni
Feb 5, 2010·Journal of Bioscience and Bioengineering·Yoon-Suk Kang, Woojun Park
Sep 9, 2015·Applied Microbiology and Biotechnology·Krisztián LacziGábor Rákhely
Mar 3, 2010·Environmental Microbiology·Yoon-Suk Kang, Woojun Park
Jun 9, 2009·International Journal of Systematic and Evolutionary Microbiology·Mario VaneechoutteLenie Dijkshoorn
Nov 22, 2014·Environmental Science and Pollution Research International·Fei HuaYan Yang
Nov 24, 2012·FEMS Microbiology Letters·Genki AkanumaMorio Ishizuka
Sep 18, 2015·Journal of Child and Family Studies·Henok ZeratsionBjørgulf Claussen
Jul 12, 2011·Metallomics : Integrated Biometal Science·Rachel Narehood Austin, John T Groves
Sep 22, 2012·Angewandte Chemie·Mélanie BordeauxJullien Drone
Jul 3, 2021·International Journal of Environmental Research and Public Health·Weina KongShiwei Wang

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