PMID: 8955389Dec 1, 1996Paper

Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA

Journal of Bacteriology
Kristian E BakerRod A Kelln

Abstract

Mutants deficient in orotate utilization (initially termed out mutants) were isolated by selection for resistance to 5-fluoroorotate (FOA), and the mutations of 12 independently obtained isolates were found to map at 79 to 80 min on the Salmonella typhimurium chromosome. A gene complementing the mutations was cloned and sequenced and found to possess extensive sequence identity to characterized genes for C4-dicarboxylate transport (dctA) in Rhizobium species and to the sequence inferred to be the dctA gene of Escherichia coli. The mutants were unable to utilize succinate, malate, or fumarate as sole carbon source, an expected phenotype of dctA mutants, and introduction of the cloned DNA resulted in restoration of both C4-dicarboxylate and orotate utilization. Further, succinate was found to compete with orotate for entry into the cell. The S. typhimurium dctA gene encodes a highly hydrophobic polypeptide of 45.4 kDa, and the polypeptide was found to be enriched in the membrane fraction of minicells harboring a dctA+ plasmid. The DNA immediately upstream of the deduced -35 region contains a putative cyclic AMP-cyclic AMP receptor protein complex binding site, thus affording an explanation for the more effective utilization of or...Continue Reading

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Citations

Feb 6, 2017·Scientific Reports·Zhaleh Hosseini, Sayed-Amir Marashi
Apr 21, 2018·Frontiers in Microbiology·Maritsa Cruz-MunozEric W Triplett
Sep 18, 2004·FEMS Microbiology Reviews·Svetlana N Yurgel, Michael L Kahn
Mar 2, 2019·PLoS Computational Biology·Colton J LloydAdam M Feist
Jan 3, 2013·Molecular Microbiology·Eleni KarinouArnaud Javelle
Jul 1, 2008·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Jul 14, 2000·Journal of Bacteriology·S YurgelM L Kahn
Jan 22, 2005·Journal of Bacteriology·Svetlana N Yurgel, Michael L Kahn
Nov 13, 1998·Journal of Bacteriology·F C BoogerdP R Jensen
Mar 23, 2021·Critical Reviews in Microbiology·Lucindo Cardoso de PinaL Caetano M Antunes
Jan 23, 2002·Biochimica Et Biophysica Acta·I G JanauschG Unden
Dec 1, 2004·EcoSal Plus·Gottfried Unden, Alexandra Kleefeld
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·D J SlotboomJ S Lolkema
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Aug 30, 2008·Journal of Molecular Biology·Yan-Feng ZhouXiao-Dong Su

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