PMID: 11339278May 8, 2001Paper

Utilization of the non-covalent fluorescent dye, NanoOrange, as a potential clinical diagnostic tool. Nanomolar human serum albumin quantitation

Journal of Chromatography. B, Biomedical Sciences and Applications
M D HarveyC D Skinner

Abstract

The commercially available dye, NanoOrange, has been investigated as a potential tool for clinical diagnostics due to its low cost, ease of use, and ability to detect nanomolar concentrations of protein. Virtually non-fluorescent in dilute aqueous solutions, NanoOrange fluorescence is enhanced by at least an order of magnitude upon non-covalent interaction with proteins. These features, coupled with the requirement for high throughput assays in the clinical laboratory has prompted the development of two orthogonal NanoOrange approaches. Human serum albumin (HSA) was used as a model protein for the development of both 96-well microplate and capillary electrophoresis laser-induced fluorescence (CE-LIF) assay formats. Dye performance in five commonly used buffers of various concentrations and pH indicated considerable flexibility in assay buffer selection, with optimal performance at pH 9.0. A salt concentration study indicated that increasing NaCl concentration generally decreases fluorescence emission and can be minimized by pre-diluting biological samples to a final salt concentration of 20-80 mM. Titration of protein with NanoOrange resulted in optimal HSA-NanoOrange complex formation utilizing 1 x and 2 x NanoOrange in the 96...Continue Reading

References

May 18, 1999·Journal of Chromatography. a·J CaslavskaW Thormann
Jan 5, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·C PetriniC Franzini
Mar 17, 2000·Biochimica Et Biophysica Acta·M PurcellH A Tajmir-Riahi
Apr 25, 2000·Hypertension·J S JensenK Borch-Johnsen
May 29, 2000·Electrophoresis·S McWhorter, S A Soper
Jun 20, 2000·Acta Oncologica·L M Pedersen, P G Sørensen
Jul 20, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·I German, R T Kennedy

❮ Previous
Next ❯

Citations

Aug 25, 2001·Analytical Biochemistry·G GeigerF Vitzthum
Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Masatake SanoHiroshi Kamimori
Jan 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kalivarathan DivakarPennathur Gautam
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E AdriaensJ P Remon
Jun 30, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A J HallJ G Babish
Sep 29, 2011·Journal of Separation Science·Keiko SumitomoKuniaki Tatsuta
Dec 1, 2006·Electrophoresis·Ana M García-CampañaHuguette Fabre
Dec 7, 2010·Journal of Applied Toxicology : JAT·Joke LenoirJean-Paul Remon
Jul 2, 2002·Analytical Chemistry·Shen Hu, Norman J Dovichi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.