Utilizing afterglow magnetization from cross-polarization magic-angle-spinning solid-state NMR spectroscopy to obtain simultaneous heteronuclear multidimensional spectra.

The Journal of Physical Chemistry. B
James R Banigan, Nathaniel J Traaseth

Abstract

The time required for data acquisition and subsequent spectral assignment are limiting factors for determining biomolecular structure and dynamics using solid-state NMR spectroscopy. While strong magnetic dipolar couplings give rise to relatively broad spectra lines, the couplings also mediate the coherent magnetization transfer via the Hartmann-Hahn cross-polarization (HH-CP) experiment. This mechanism is used in nearly all backbone assignment experiments for carrying out polarization transfer between (1)H, (15)N, and (13)C. In this Article, we describe a general spectroscopic approach to use the residual or "afterglow" magnetization from the (15)N to (13)C selective HH-CP experiment to collect a second multidimensional heteronuclear data set. This approach allowed for the collection of two commonly used sequential assignment experiments (2D NCA and NCO or 3D NCACX and NCOCX) at the same time. Our "afterglow" technique was demonstrated with uniformly [(13)C,(15)N] and [1,3-(13)C] glycerol-labeled ubiquitin using instrumentation available on all standard solid-state NMR spectrometers configured for magic-angle-spinning. This method is compatible with several other sensitivity enhancement experiments and can be used as an isotop...Continue Reading

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Citations

May 17, 2013·The Journal of Chemical Physics·T Gopinath, Gianluigi Veglia
Mar 6, 2013·Angewandte Chemie·Kevin J DonovanLucio Frydman
Feb 3, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nishit GoradiaRamadurai Ramachandran
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Jan 25, 2019·Magnetic Resonance in Chemistry : MRC·Pau Nolis, Teodor Parella
Jun 10, 2020·Journal of Biomolecular NMR·Jianping LiNathaniel J Traaseth
Mar 4, 2021·Accounts of Chemical Research·Tata GopinathGianluigi Veglia
Jun 15, 2017·Journal of the American Chemical Society·Daryl GoodVladimir Ladizhansky
Sep 5, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·Ēriks KupčeTim D W Claridge

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