Utilizing multiparametric flow cytometry in the diagnosis of patients with primary plasma cell leukemia.

American Journal of Hematology
Laura A EvansW I Gonsalves

Abstract

The diagnosis of primary plasma cell leukemia (pPCL) has been made by quantifying circulating plasma cells (cPCs) morphologically on a peripheral blood (PB) smear. However, this technique is not sufficiently sensitive. Multiparametric flow cytometry (MFC) provides a readily available and highly sensitive method to identify and quantify cPCs that could complement PB smear assessment. However, an optimal quantitative cutoff for cPCs by MFC to identify pPCL has not been established. Thus, a total of 591 patients newly diagnosed multiple myeloma (NDMM) patients who had their PB samples evaluated morphologically by PB smear, and immunophenotypically by MFC prior to beginning therapy were evaluated. The presence of ≥200 cPCs/μL by MFC (N = 25 or 5% of the total population) was chosen to identify patients with ≥5% cPCs by PB smear with a specificity of 99% and a sensitivity of 77%. For patients with ≥200 cPCs/μL by MFC compared to the remainder of the cohort, the median Time to next therapy (TTNT) was 18 vs 30 months and the median OS was 38 vs 70 months respectively. Thus, MFC assessment of PB can be utilized in conjunction with the morphological assessment of a PB smear to aid in improving the identification of pPCL among NDMM patie...Continue Reading

References

Dec 1, 1987·The American Journal of Medicine·P Noel, R A Kyle
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Aug 13, 2014·British Journal of Haematology·Wilson I GonsalvesShaji K Kumar
Apr 27, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bruno RoyerJean Pierre Marolleau
Nov 8, 2016·Leukemia & Lymphoma·Roberto MinaAntonio Palumbo
Mar 4, 2017·Haematologica·Miquel GranellUNKNOWN GEMMAC (Grup per l’estudi del mieloma i l’amiloïdosi de Catalunya)
Mar 9, 2017·Revista brasileira de hematologia e hemoterapia·Aleyde Diniz LoureiroMihoko Yamamoto
Mar 25, 2019·Expert Review of Hematology·Pellegrino MustoFiorella D'Auria

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Citations

Feb 11, 2021·Blood Cancer Journal·Sherilyn A TuazonPaul G Richardson
Jun 27, 2021·Cancer Treatment and Research Communications·A VisramW I Gonsalves

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