Utilizing Organoid and Air-Liquid Interface Models as a Screening Method in the Development of New Host Defense Peptides.

Frontiers in Cellular and Infection Microbiology
Ka-Yee Grace ChoiRobert E W Hancock

Abstract

Host defense peptides (HDPs), also known as antimicrobial peptides, are naturally occurring polypeptides (~12-50 residues) composed of cationic and hydrophobic amino acids that adopt an amphipathic conformation upon folding usually after contact with membranes. HDPs have a variety of biological activities including immunomodulatory, anti-inflammatory, anti-bacterial, and anti-biofilm functions. Although HDPs have the potential to address the global threat of antibiotic resistance and to treat immune and inflammatory disorders, they have yet to achieve this promise. Indeed, there are several challenges associated with bringing peptide-based drug candidates from the lab bench to clinical practice, including identifying appropriate indications, stability, toxicity, and cost. These challenges can be addressed in part by the development of innate defense regulator (IDR) peptides and peptidomimetics, which are synthetic derivatives of HDPs with similar or better efficacy, increased stability, and reduced toxicity and cost of the original HDP. However, one of the largest gaps between basic research and clinical application is the validity and translatability of conventional model systems, such as cell lines and animal models, for scre...Continue Reading

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Citations

Nov 25, 2020·The American Journal of Pathology·Alexandra McCarronMartin Donnelley
Jan 27, 2021·NPJ Biofilms and Microbiomes·Bing Catherine WuRobert E W Hancock
Mar 12, 2021·Frontiers in Cellular and Infection Microbiology·Christophe Beloin, Diane McDougald
May 5, 2021·Advanced Biology·Sarah L BarronRóisín M Owens
Jun 30, 2021·Nature Reviews. Microbiology·Robert E W HancockEvan F Haney
Jun 14, 2021·European Biophysics Journal : EBJ·Ilaria TortorellaFrancesco Morena
Sep 8, 2021·Frontiers in Immunology·Laurine C RijsbergenRik L de Swart
Oct 7, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dave Anuj LagowalaDeok-Ho Kim

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Methods Mentioned

BETA
chip
biosensors
microsensors
chips
RNA-Seq
xenograft
genetic modification
transgenic
glucosylation
fluorescence microscopy

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