Utilizing prestin as a predictive marker for the early detection of outer hair cell damage

American Journal of Otolaryngology
Murat DoganBuket Demirci

Abstract

To evaluate prestin as a biomarker for the identification of early ototoxicity. Rats (n = 47) were randomly assigned to five groups: low-dose (LAG) or high-dose (HAG) amikacin (200 and 600 mg/kg/day, respectively, for 10 days), low-dose (LCIS)or high-dose (HCIS) cisplatin (single doses of 5 and 15 mg/kg, respectively, for 3 days), and control (n = 8). At the end of the experiment, measurement of distortion product-evoked otoacoustic emissions (DPOAE) were performed to evaluate hearing, then blood samples and both ear tissues were collected under anesthesia. Prestin levels were determined by ELISA. Cochlear damage was evaluated histologically using a 4-point scoring system. The mean serum prestin levels were 377.0 ± 135.3, 411.3 ± 73.1, 512.6 ± 106.0, 455.0 ± 74.2 and 555.3 ± 47.9 pg/ml for control, LCIS, HCIS, LAG and HAG groups, respectively. There was significant difference between prestin levels of Control-LCIS-HCIS groups (p = 0.031) and prestin levels of Control-LAG-HAG groups (p = 0.003). There were also significant differences in prestin levels between the low- and high-dose cisplatin and amikacin groups (p = 0.028 and p = 0.011, respectively). Each group had significantly lower DPOAE results at 4, 6 and 8 kHz than contr...Continue Reading

Citations

Jun 22, 2019·International Journal of Toxicology·David V GauvinTheodore J Baird
Dec 4, 2019·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Erin Mulry, Kourosh Parham
Apr 27, 2021·Ear and Hearing·Eleftheria IliadouAthanasios Bibas
Oct 26, 2021·Ear, Nose, & Throat Journal·Ruwaa SamarraiKourosh Parham

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