Utilizing T-cell Activation Signals 1, 2, and 3 for Tumor-infiltrating Lymphocytes (TIL) Expansion: The Advantage Over the Sole Use of Interleukin-2 in Cutaneous and Uveal Melanoma

Journal of Immunotherapy
René J TaveraCara Haymaker

Abstract

In this study, we address one of the major critiques for tumor-infiltrating lymphocyte (TIL) therapy-the time needed for proper expansion of a suitable product. We postulated that T-cell receptor activation in the first phase of expansion combined with an agonistic stimulation of CD137/4-1BB and interleukin-2 would favor preferential expansion of CD8 TIL. Indeed, this novel 3-signal approach for optimal T-cell activation resulted in faster and more consistent expansion of CD8CD3 TIL. This new method allowed for successful expansion of TIL from cutaneous and uveal melanoma tumors in 100% of the cultures in <3 weeks. Finally, providing the 3 signals attributed to optimal T-cell activation led to expansion of TIL capable of recognizing their tumor counterpart in cutaneous and uveal melanoma. This new methodology for the initial phase of TIL expansion brings a new opportunity for translation of TIL therapy in challenging malignancies such as uveal melanoma.

References

Apr 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark E DudleySteven A Rosenberg
Apr 22, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michal J BesserJacob Schachter
Oct 4, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Laszlo G RadvanyiPatrick Hwu
Sep 27, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M MaioP A Ascierto
Dec 5, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jessica Ann ChaconLaszlo Radvanyi
Apr 1, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sanja StevanovićChristian S Hinrichs
Mar 24, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rikke AndersenInge Marie Svane
Oct 26, 2016·Journal for Immunotherapy of Cancer·MacLean HallAmod A Sarnaik
Sep 28, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Donastas Sakellariou-ThompsonChantale Bernatchez

❮ Previous
Next ❯

Citations

Sep 19, 2019·European Journal of Immunology·Caitlin A CreasyAung Naing
Oct 12, 2019·Cancer Immunology, Immunotherapy : CII·Donastas Sakellariou-ThompsonChantale Bernatchez
Oct 1, 2020·Cancers·Fabiana MalloneAntonietta Moramarco
May 31, 2020·Cancer Immunology, Immunotherapy : CII·Morten NielsenNiels Junker
Jun 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie-Andrée ForgetChantale Bernatchez
Oct 13, 2019·Seminars in Immunology·Jean-Philippe BastienDenis Claude Roy
Mar 9, 2021·Frontiers in Oncology·Anaïs Jiménez-ReinosoLuis Álvarez-Vallina
May 1, 2021·Cancers·Mariarosaria MarsegliaMichela Croce

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
Assay
biopsy

Clinical Trials Mentioned

NCT00338377

Software Mentioned

GraphPah prism
CTL Immunospot Reader
FlowJo
GraphPad

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Related Papers

Progress in Clinical and Biological Research
T L WhitesideR B Herberman
Polskie Archiwum Medycyny Wewnętrznej
W Matysiak
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
T DorvalP Pouillart
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Richard W JosephLaszlo G Radvanyi
© 2022 Meta ULC. All rights reserved