Utrophins compensate for Dp71 absence in mdx3cv in adhered platelets

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
Doris CerecedoDalila Martínez-Rojas

Abstract

Platelet adhesion is a critical step due to its hemostatic role in stopping bleeding after vascular damage. Short dystrophins are the most abundant dmd gene products in nonmuscle tissues, and in association with cytoskeleton proteins contribute to their intrinsic function; while utrophins are dystrophin-homologous related family proteins with structural and functional similarities. We previously demonstrated the presence of Dp71 isoforms, utrophins, and various dystrophin-associated proteins and their participation in cytoskeleton re-organization, filopodia and lamellipodia extension, and in centralizing cytoplasmic granules during the adhesion process of human platelets. To evaluate the morphologic changes and actin-based structures of mdx(3cv) platelets during the adhesion process, we compared the topographic distribution of Dp71d/Dp71Delta110(m) and dystrophin-associated protein in adhered platelets from dystrophic mdx(3cv) mouse. By confocal microscopy, we showed that absence of Dp71 isoforms in platelets from this animal model disrupted dystrophin-associated protein expression and distribution without modifying the platelet morphology displayed during the glass-adhesion process. By immunoprecipitation assays, we proved tha...Continue Reading

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Citations

Jul 14, 2010·Journal of Biomedicine & Biotechnology·Caroline Perronnet, Cyrille Vaillend
Sep 14, 2013·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Doris Cerecedo
Nov 23, 2011·Molecular Neurobiology·Ramin TadayoniBulmaro Cisneros

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