UV-C inactivation in Escherichia coli is affected by growth conditions preceding irradiation, in particular by the specific growth rate

Journal of Applied Microbiology
M Bucheli-WitschelT Egli

Abstract

The objective was to analyse the impact of growth conditions, in particular of the specific growth rate, on the resistance of Escherichia coli towards UV-C irradiation. Escherichia coli K12 wild-type bacteria (and in some experiments also a mutant not expressing RpoS, the global regulator of the general stress response; rpoS(-) mutant) were cultivated either in batch culture until stationary phase was reached or in continuous culture at different specific growth rates (μ) and then irradiated with UV-C light. Inactivation was determined by plating. The specific growth rate had a profound effect on UV-C resistance. Stationary phase or very slowly growing cells (0≤μ<0·1 h(-1)) as well as fast-growing cells exhibited a high resistance compared to bacteria growing at an intermediate rate (between 0·2 and 0·4 h(-1) ). The rpoS(-) mutant was more susceptible to UV irradiation than the wild-type when obtained from stationary phase, while mutant cells from continuous culture (μ=0·2 h(-1)) revealed a UV-C resistance similar to the wild-type grown under the same conditions. Antecedent growth conditions determine the physiological state of bacteria including the resistance towards UV-C irradiation. In particular, the specific growth rate w...Continue Reading

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Citations

Sep 25, 2012·Applied and Environmental Microbiology·E GayánS Condón
Sep 4, 2012·Applied and Environmental Microbiology·Lauren M SassoubreAlexandria B Boehm
Aug 3, 2014·Food Microbiology·George C PaoliChandi Wijey
Nov 21, 2012·Journal of Photochemistry and Photobiology. B, Biology·J J Vélez-ColmenaresE Nebot
Feb 18, 2015·Journal of Applied Microbiology·P A MaracciniA B Boehm
Dec 24, 2013·International Journal of Food Microbiology·E GayánS Condón
Feb 24, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Andréia Miho Morishita Harada, Maristela Silva Nascimento
Dec 1, 2011·EcoSal Plus·Regine Hengge
Nov 5, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Momo OtakeJun Hidema

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