PMID: 40230Aug 1, 1979

UV-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA

Proceedings of the National Academy of Sciences of the United States of America
E A WaldsteinR B Setlow

Abstract

We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with UV doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after UV irradiation. The calf thymus endonuclease is different from other mammalian UV-endonucleases so far described in that it seems to be dimer specific. The enzyme is stimulated by Mg2+ and is inactive in the presence of EDTA. It binds to UV-irradiated DNA-Sepharose from which it is released by low concentrations of KCl. Gel filtration data indicate that the endonuclease may belong to a high molecular weight protein or protein complex. The enzyme is very labile and freezing increases its lability.

References

Sep 30, 1982·Biochemical and Biophysical Research Communications·A O OkoroduduM W Lambert
Dec 1, 1983·Mutation Research·E J KatzM A Sirover
May 2, 1985·European Journal of Biochemistry·D E HellandK Kleppe
Jan 1, 1986·Radiation and Environmental Biophysics·U Hagen
May 31, 1984·Biochemical and Biophysical Research Communications·A TriantosA Trakatellis
May 1, 1988·Journal of Oral Pathology·N M Boyd, P C Reade

Citations

May 16, 1975·Biochimica Et Biophysica Acta·S Bacchetti, R Benne
Oct 1, 1977·Mutation Research·H SlorE C Friedberg
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E Seeberg
Jul 15, 1977·Biochimica Et Biophysica Acta·G W TeeborF F Becker
Jun 1, 1975·European Journal of Biochemistry·D J Arndt-JovinM Marquardt
Aug 7, 1972·Biochemical and Biophysical Research Communications·S BacchettiG Veldhuisen
Jun 14, 1974·Biochimica Et Biophysica Acta·J L van Lancker, T Tomura
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E A WaldsteinR Ben-Ishai
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Apr 1, 1968·Virology·R T Espejo, E S Canelo

Related Concepts

Structure of Calf of Leg
Endonuclease
Neoplasm of Uncertain or Unknown Behavior of Thymus
Chromatography
Cattle calf (organism)
Base Excision Repair
Bacteriophages
Bos taurus
Disease of Thymus Gland
Thymic Tissue

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