UV excitation thermal lens microscope for sensitive and nonlabeled detection of nonfluorescent molecules

Analytical Chemistry
Shinichiro HikiTakehiko Kitamori

Abstract

An ultrasensitive and nonlabeled detection method of nonfluorescent molecules on a microchip was developed by realizing a thermal lens microscope (TLM) with a 266-nm UV pulsed laser as an excitation light source (UV-TLM). Pulsed laser sources have advantages over continuous-wave laser sources in more compact size and better wavelength tuning, which are important for microchip-based analytical systems. Their disadvantage is difficulty in applying a lock-in amplifier due to the high (>10(4)) duty ratio of pulse oscillation. To overcome this problem, we realized a quasi-continuous-wave excitation by modulating the pulse trains at approximately 1 kHz and detecting the synchronous signal with a lock-in amplifier. The optimum pulse repetition frequency was obtained at 80 kHz, which was reasonable considering thermal equilibrium time. Furthermore, a permissible flow velocity in the range of 6.6-19.8 mm/s was found to avoid sensitivity decrease due to photochemical reactions and thermal energy dissipation. Under these conditions, we detected adenine aqueous solutions on a fused-silica microchip without labeling and obtained a sensitivity that was 350 times higher than that in a spectrophotometric method. The sensitivity was enough for ...Continue Reading

References

Jan 15, 2005·Analytical Chemistry·Manabu TokeshiTakehiko Kitamori
Feb 1, 2005·Analytical Chemistry·Akihide HibaraTakehiko Kitamori

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Citations

Apr 9, 2008·Analytical and Bioanalytical Chemistry·Kazuma MawatariTakehiko Kitamori
Nov 5, 2008·Analytical and Bioanalytical Chemistry·Philipp Schulze, Detlev Belder
Jun 11, 2009·Analytical and Bioanalytical Chemistry·Jilska M Perera, Geoffrey W Stevens
Apr 24, 2010·Analytical Chemistry·Patty J DennisJames W Jorgenson
Mar 21, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yo TanakaTakehiko Kitamori
Feb 13, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuhiro SetaTakehiko Kitamori
Nov 14, 2014·Critical Reviews in Analytical Chemistry·Mingqiang Liu, Mladen Franko
Apr 18, 2017·Applied Optics·Nader ShokoufiKazem Kargosha
Jul 12, 2014·Applied Spectroscopy·Odon S ArésteguiNelson G C Astrath
Apr 9, 2008·Electrophoresis·Ryo AnrakuTakehiko Kitamori
Jul 14, 2010·The Analyst·Mikhail N SlipchenkoJi-Xin Cheng
Jul 1, 2011·Laser & Photonics Reviews·Shuhua YueJi-Xin Cheng
Dec 24, 2011·Applied Optics·Oluwatosin O DadaNorman J Dovichi
Dec 13, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Miki IsodaAkira Harata
Jan 1, 2008·Microfluidics and Nanofluidics·Hamish C Hunt, James S Wilkinson
Jan 18, 2014·Electrophoresis·Christopher L CassanoZ Hugh Fan
Nov 25, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Miki IsodaAkira Harata

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