UV irradiation and autoclave treatment for elimination of contaminating DNA from laboratory consumables

Forensic Science International. Genetics
Lisa A GefridesRoger Kahn

Abstract

Laboratories employ various approaches to ensure that their consumables are free of DNA contamination. They may purchase pre-treated consumables, perform quality control checks prior to casework, and use in-house profile databases for contamination detection. It is better to prevent contamination prior to DNA typing than identify it after samples are processed. To this end, laboratories may UV irradiate or autoclave consumables prior to use but treatment procedures are typically based on killing microorganisms and not on the elimination of DNA. We report a systematic study of UV and autoclave treatments on the persistence of DNA from saliva. This study was undertaken to determine the best decontamination strategy for the removal of DNA from laboratory consumables. We have identified autoclave and UV irradiation procedures that can eliminate nanogram quantities of contaminating DNA contained within cellular material. Autoclaving is more effective than UV irradiation because it can eliminate short fragments of contaminating DNA more effectively. Lengthy autoclave or UV irradiation treatments are required. Depending on bulb power, a UV crosslinker may take a minimum of 2h to achieve an effective dose for elimination of nanogram qu...Continue Reading

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Citations

Jun 19, 2013·Croatian Medical Journal·Branka GrškovićGordan Mršić
Jun 8, 2014·BMC Genomics·S Timothy MotleyMark W Eshoo
Mar 15, 2013·Forensic Science International. Genetics·Jan Cemper-KiesslichFranz Neuhuber
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Sep 6, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Lihua LanDezhao Liu
Mar 8, 2021·Environmental Science and Pollution Research International·Jun LvShan Li
Mar 14, 2021·Forensic Science International. Genetics·Ana NeckovicAnnalisa Durdle

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