PMID: 16512414Mar 4, 2006Paper

UV light-emitting diode-induced fluorescence detection combined with online sample concentration techniques for capillary electrophoresis

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Yen-Sheng ChangCheng-Huang Lin

Abstract

The application of an ultraviolet (UV) light-emitting diode (LED) to on-line sample concentration/fluorescence detection in capillary electrophoresis (CE) is described. The utility of a UV-LED (peak emission wavelength at 380 nm, approximately 2 mW) for fluorescence detection was demonstrated by examining both a naturally fluorescent (riboflavin) compound and a nonfluorescent compound (tryptophan), respectively. The detection limit for riboflavin was determined to be 0.2 ppm by the normal MEKC mode, which was improved to 3-7 ppb when dynamic pH-junction technique was applied. On the other hand, the detection limit of the tryptophan derivative was determined to be 1.5 ppm using the MEKC mode, which was improved to 3 ppb when the sweeping-MEKC mode was applied. In an analysis of an actual sample, the concentrations of riboflavin in beer, and tryptophan in urine and milk samples were determined, respectively.

References

May 29, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·I MiksíkZ Deyl
Feb 24, 2001·Journal of Chromatography. a·Z K Shihabi
Jun 22, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A MarwahH Lardy
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Mar 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·Attila L MajorMartin J C van Gemert
Jan 22, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·An-Kai Su, Cheng-Huang Lin

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Citations

Sep 12, 2006·Nucleic Acids Research·Sarah BlairFranco Cerrina
Nov 24, 2007·Journal of Chromatography. a·Steven L SimpsonShigeru Terabe
Oct 19, 2011·Journal of Separation Science·Artaches A KazarianMichael C Breadmore
Dec 13, 2006·Electrophoresis·Dan XiaoXiupei Yang
Dec 17, 2008·Electrophoresis·Dan XiaoMartin M F Choi

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