PMID: 9188274Jun 1, 1997Paper

UV light-induced crosslinking of the complementary strands of plasmid pUC19 DNA restriction fragments

Photochemistry and Photobiology
S Pospísilová, J Kypr

Abstract

Restriction fragments of pUC19 DNA were irradiated by various doses of UV light and analyzed by denaturing (alkaline) agarose gel electrophoresis. The irradiation generated retarded species whose mobility indicated two crosslinked DNA strands. Quantitative analysis of the experimental data provided an empirical equation relating the fraction of crosslinked DNA molecules to their length and to the dose of their irradiation by UV light. This equation can be used to predict the crosslinking behavior of pUC19-like DNA molecules whose primary structures do not much differ from a random nucleotide sequence. The amount of interstrand crosslinks increased with the (A+T) content of the pUC19 DNA fragments but the dependence was not clear-cut to indicate that oligonucleotide composition of DNA played a significant role as well.

References

Jul 18, 1967·Biochimica Et Biophysica Acta·V R Glisin, P Doty
Nov 1, 1994·Journal of Molecular Evolution·G P Holmquist
Jun 1, 1994·Journal of Biomolecular Structure & Dynamics·J KyprM Vorlícková
Jan 1, 1996·Nucleic Acids Research·P Rodriguez-ToméT P Flores
Jun 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·J MARMUR, L GROSSMAN

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Citations

May 23, 2001·Archives of Biochemistry and Biophysics·K NejedlýJ Kypr
Oct 11, 2003·Biotechnology Advances·V P GrishkoB R Glick
Jan 13, 2005·Nucleic Acids Research·Karel NejedlýJaroslav Kypr
Feb 24, 2006·Journal of Photochemistry and Photobiology. B, Biology·Andrzej WojcikGünter Obe
Jun 11, 2015·The Journal of Physical Chemistry. B·Janusz RakŁukasz Golon

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