UV resonance raman investigation of electronic transitions in alpha-helical and polyproline II-like conformations.

The Journal of Physical Chemistry. B
Bhavya SharmaSanford A Asher

Abstract

UV resonance Raman (UVRR) excitation profiles and Raman depolarization ratios were measured for a 21-residue predominantly alanine peptide, AAAAA(AAARA) 3A (AP), excited between 194 and 218 nm. Excitation within the pi-->pi* electronic transitions of the amide group results in UVRR spectra dominated by amide vibrations. The Raman cross sections and excitation profiles provide information about the nature of the electronic transitions of the alpha-helix and polyproline II (PPII)-like peptide conformations. AP is known to be predominantly alpha-helical at low temperatures and to take on a PPII helix-like conformation at high temperatures. The PPII-like and alpha-helix conformations show distinctly different Raman excitation profiles. The PPII-like conformation cross sections are approximately twice those of the alpha-helix. This is due to hypochromism that results from excitonic interactions between the NV 1 transition of one amide group with higher energy electronic transitions of other amide groups, which decreases the alpha-helical NV 1 (pi-->pi*) oscillator strengths. Excitation profiles of the alpha-helix and PPII-like conformations indicate that the highest signal-to-noise Raman spectra of alpha-helix and PPII-like conforma...Continue Reading

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Citations

Oct 23, 2009·Biochemistry·Kan XiongSanford A Asher
Feb 18, 2012·Chemical Reviews·Sulayman A OladepoIgor K Lednev
Mar 18, 2011·The Journal of Physical Chemistry. B·Bhavya Sharma, Sanford A Asher
Jan 13, 2012·Biophysical Chemistry·Kan XiongSanford A Asher
Apr 24, 2010·Applied Spectroscopy·David D TuschelSanford A Asher
Aug 3, 2017·The Review of Scientific Instruments·Martin HöhlMerve Meinhardt-Wollweber
Aug 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martynas TalaikisGediminas Niaura
Aug 7, 2012·The Journal of Physical Chemistry. B·Cynthia V Pagba, Bridgette A Barry
Mar 8, 2011·The Journal of Physical Chemistry Letters·Sulayman A OladepoSanford A Asher

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