UVA activation of N-dialkylnitrosamines releasing nitric oxide, producing strand breaks as well as oxidative damages in DNA, and inducing mutations in the Ames test

Mutation Research
S Arimoto-KobayashiKeiko Yakushi

Abstract

We investigated the photo-mutagenicity and photo-genotoxicity of N-dialkylnitrosamines and its mechanisms of UVA activation. With simultaneous irradiation of UVA, photo-mutagenicity of seven N-dialkylnitrosamines was observed in Ames bacteria (Salmonella typhimurium TA1535) in the absence of metabolic activation. Mutagenicity of pre-irradiated N-dialkylnitrosamines was also observed with S. typhimurium hisG46, TA100, TA102 and YG7108 in the absence of metabolic activation. UVA-mediated mutation with N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) decreased by adding either the NO or OH radical scavenger. When superhelical DNA was irradiated with N-dialkylnitrosamines, nicked circular DNA appeared. Ten N-dialkylnitrosamines examined produced strand breaks in the treated DNA in the presence of UVA. The level of single-strand breaks in phiX174 DNA mediated by N-nitrosomorpholine (NMOR) and UVA decreased by adding either a radical scavenger or superoxide dismutase. When calf thymus DNA was treated with N-dialkylnitrosamines (NDMA, NDEA, NMOR, N-nitrosopyrrolidine (NPYR) and N-nitrosopiperidine (NPIP)) and UVA, the ratio of 8-oxodG/dG in the DNA increased. Action spectra were obtained to determine if nitrosamine acts ...Continue Reading

References

Jan 1, 1987·Free Radical Biology & Medicine·T A GroverL H Piette
Nov 1, 1970·Tetrahedron·W LijinskyJ Loo
Apr 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K R BrainS D Gettings
Dec 13, 2000·Mutation Research·K Mortelmans, E Zeiger
Feb 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Georg T WondrakElaine L Jacobson
May 29, 2007·Cancer Investigation·Christian C Abnet
Dec 7, 2007·Chemical Research in Toxicology·Stephen S Hecht
Dec 5, 2009·Carcinogenesis·Paolo Boffetta
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mohamad SleimanHugo Destaillats

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