PMID: 2498481Feb 1, 1989Paper

UVA-induced binding of 8-methoxypsoralen to cells of Saccharomyces cerevisiae: separation and characterization of DNA photoadducts

Journal of Photochemistry and Photobiology. B, Biology
M Bankmann, M Brendel

Abstract

We present methods for the determination of UVA-induced binding of 8-methoxypsoralen (8-MOP) to nucleic acids and protein and for a quantitative assay of radioactively labelled 8-MOP plus UVA induced DNA photoproducts in the yeast Saccharomyces cerevisiae. For the dose range up to 60 kJ m-2, with a wild-type survival of 1% or higher, binding to DNA is 100-fold and to RNA 10- to 20-fold more efficient than that to protein. Between 20% and 65% of the 8-MOP binds to macromolecules that are neither nucleic acids nor protein. The number of DNA-bound 8-MOP molecules for the haploid genome rises from 14 (unirradiated control) to 349 at the highest UVA exposure dose (60 kJ m-2). Gel chromatography reveals three types of DNA thymine photoproduct, the pyrone-side monoadducts, the furan-side monoadducts and the diadducts. Among these, pyrone-side monoadducts always constitute the smallest fraction, regardless of whether the treatment is with in vitro or in vivo 8-MOP plus UVA.

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Citations

Feb 9, 2006·Chemical Reviews·David M NollPaul S Miller
Dec 1, 1989·Photochemistry and Photobiology·D Averbeck
Jul 12, 2012·Photochemistry and Photobiology·Jean CadetThierry Douki
Dec 1, 1990·Current Genetics·J A Henriques, M Brendel
Jan 1, 1992·Journal of Photochemistry and Photobiology. B, Biology·G RontóG Csik

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