UVA photoirradiation of anhydroretinol--formation of singlet oxygen and superoxide

Toxicology and Industrial Health
J J YinP P Fu

Abstract

Anhydroretinol is a metabolite of vitamin A (retinol) and a major photodecomposition product of retinyl palmitate and retinyl acetate. Anhydroretinol is biologically active, inducing cell death in lymphoblastoid cells, prevention of N-methyl-N-nitrosourea-induced mammary cancer, and inhibition of cell growth in lymphocytes. We have previously determined that photoirradiation of anhydroretinol in the presence of a lipid, methyl linoleate, with UVA light-induced lipid peroxidation. In the present study, electron spin resonance (ESR) spin-trap techniques were employed to explore the mechanism of lipid peroxidation initiation. Irradiation of anhydroretinol by UVA in the presence of 2,2,6,6-tetramethylpiperidine (TEMP), a specific probe for singlet oxygen, resulted in the formation of TEMPO, indicating that singlet oxygen was generated. During photoirradiation in the presence of 5,5-dimethyl N-oxide pyrroline (DMPO), a specific probe for superoxide, ESR signals for DMPO-OOH were formed, and these signals were quenched by superoxide dismutase. The involvement of singlet oxygen on the induction of lipid peroxidation was also evidenced by the observation that lipid peroxidation was inhibited by sodium azide and enhanced by deuterium ox...Continue Reading

References

Dec 13, 1991·Science·J BuckU Hämmerling
May 1, 1997·Chemical Research in Toxicology·E R Stadtman, B S Berlett
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·M Tokita, M Morita
Jul 25, 2000·Methods in Enzymology·R M Tyrrell
Apr 11, 2001·Experimental Gerontology·R A FloydK Hensley
Sep 15, 2001·Archives of Biochemistry and Biophysics·L E LambJ D Simon
Nov 23, 2006·Chemical Research in Toxicology·Nan MeiPeter P Fu
Jun 20, 2007·Photochemistry and Photobiology·Peter P FuWayne G Wamer

❮ Previous
Next ❯

Citations

Apr 21, 2012·Photochemistry and Photobiology·Małgorzata RóżanowskaBartosz Różanowski
Nov 19, 2013·Journal of the American Academy of Dermatology·Rebecca JansenHenry W Lim
Mar 29, 2014·Journal of Food and Drug Analysis·Peter P FuHongtao Yu
Jul 27, 2010·Advanced Drug Delivery Reviews·Nathan Bushue, Yu-Jui Yvonne Wan
Aug 10, 2010·Journal of the American Academy of Dermatology·Steven Q WangHenry W Lim
Mar 12, 2011·Photodermatology, Photoimmunology & Photomedicine·Mark E Burnett, Steven Q Wang
Oct 14, 2017·International Journal of Toxicology·Wilbur Johnson
Oct 12, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Wolfgang BäumlerTim Maisch
Sep 13, 2013·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Peter P FuHsiu-Mei Chiang
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.