PMID: 11328722May 1, 2001Paper

Uveal melanoma: mean of the longest nucleoli measured on silver-stained sections

Investigative Ophthalmology & Visual Science
A Moshari, I W McLean

Abstract

To summarize and compare the various histologic methodologies for using nucleoli to assess the malignant potential of uveal melanoma. This was an observational series of 100 samples of uveal melanoma in which histologic sections were studied. The cases were selected so that approximately half (n = 49) of the tumors were from patients who had died of metastatic malignant melanoma. The 51 remaining tumors were from patients who had survived at least 9 years without development of metastasis. Central sections from the uveal melanomas were stained using the colloidal silver nitrate stain for nucleolar organizing regions (AgNOR). These were compared with an adjacent hematoxylin and eosin (H&E)-stained section. A light microscope with a micrometer inset into the eyepiece (x10) was used at a final magnification of x1000 under oil immersion to measure the length of the nucleolus along the longest axis and the width perpendicular to that axis. From at least twenty cells selected from random fields throughout the tumor, the mean of the 10 longest and widest nucleoli (MLN) was calculated. Seven samples had to be discarded because the nucleoli were unmeasurable. T-tests and Cox proportional hazard regression analysis indicated that the MLN...Continue Reading

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