Uveal melanomas express vascular endothelial growth factor and basic fibroblast growth factor and support endothelial cell growth

The British Journal of Ophthalmology
S R BoydI A Cree

Abstract

Tumour microvascularity is a significant determinant of prognosis for a large number of different tumours, including uveal melanoma. The development of blood vessels within these and other tumours is partly controlled by soluble pro-angiogenic cytokines, of which basic fibroblast growth factor (bFGF) and vascular endothelial growth factor-A (VEGF) are the best described. Because VEGF has been inconsistently found within uveal melanomas and bFGF is described as an autocrine growth factor in cutaneous melanoma, the authors looked at the expression of these cytokines in uveal melanomas using immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). The cross talk between uveal melanoma cells and endothelial cells was then assessed in an in vitro co-culture model. While most tumour cells expressed bFGF at the protein level by immunohistochemistry (89%), relatively few (22%) expressed VEGF, and this was of limited extent. All 20 tumours tested by RT-PCR contained mRNA for both bFGF and VEGF. Co-culture experiments using an ATP based bioassay showed that uveal melanomas could support the growth of a rat brain endothelial cell line (GPNT) and human umbilical vein endothelial cells (HUVEC), and that this could ...Continue Reading

References

May 1, 1992·Survey of Ophthalmology·D M AlbertJ K Willson
Jan 3, 1990·Journal of the National Cancer Institute·J Folkman
Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Jul 1, 1991·The Journal of Pathology·M E Fallowfield, M G Cook
Nov 1, 1994·Journal of Bioluminescence and Chemiluminescence·P E AndreottiH W Bruckner
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ParangiD Hanahan
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D HanahanJ Arbeit
Mar 1, 1997·Histopathology·S B Fox
Mar 1, 1997·The British Journal of Ophthalmology·A J FossS Lightman
Dec 13, 1997·Anti-cancer Drugs·N MyattP N Plowman
Feb 17, 1998·Virchows Archiv : an International Journal of Pathology·E TolnayK M Müller
May 29, 1998·Histopathology·K G MorganH Buckley
Jul 29, 1998·Methods in Molecular Biology·I A Cree
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H Marti, W Risau
Mar 2, 1999·Journal of the National Cancer Institute·T MäkitieT Kivelä
May 26, 1999·Annals of Plastic Surgery·D S SteinbrechM T Longaker
Aug 27, 1999·Biochemical and Biophysical Research Communications·D A SimpsonA W Stitt
Jun 30, 2000·The British Journal of Ophthalmology·T G SheidowJ G Heathcote
Oct 1, 1960·The Journal of Pathology and Bacteriology·J S BECK, B E NORDIN

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Citations

May 15, 2003·The Surgical Clinics of North America·Devron H Char
May 12, 2010·American Journal of Clinical Oncology·Kristan D GuenterbergWilliam E Carson
Mar 16, 2012·Retina·Roman DunavoelgyiUrsula Schmidt-Erfurth
Mar 22, 2014·Clinical & Experimental Metastasis·Armelle CalipelFrédéric Mouriaux
Nov 30, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Shih-Chun ChaoHung-Yu Lin
Apr 10, 2010·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Sonia N YeungDavid A Maberley
Jan 28, 2009·Survey of Ophthalmology·Nathan M Radcliffe, Paul T Finger
Jan 30, 2008·Cancer Treatment Reviews·Pierre L TriozziArun D Singh
Jan 17, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jens LindegaardSteffen Heegaard
Nov 29, 2011·Veterinary Ophthalmology·Daniel R BinderWilliam R Huckle
Jan 18, 2006·Clinical Biochemistry·Jian-Ping Tong, Yu-Feng Yao
Dec 7, 2010·Progress in Retinal and Eye Research·Martine J JagerMichele C Madigan
May 18, 2006·Acta Ophthalmologica Scandinavica·Eoin GuerinBertil Damato
Aug 17, 2006·Seminars in Ophthalmology·Mark I Rosenblatt, Dimitri T Azar
Oct 11, 2011·Clinical & Experimental Metastasis·Alexandra GiatromanolakiMichael I Koukourakis
Sep 13, 2006·Current Eye Research·Irene Christa NottingGabriel van der Pluijm
Jun 20, 2019·Cancers·Florian CastetJosep Maria Piulats
Feb 1, 2019·Medicine·Yong ChengJianhong Liang
Nov 25, 2018·Archives of Pharmacal Research·Dong Hyun JoJeong Hun Kim
May 10, 2021·Journal of Experimental & Clinical Cancer Research : CR·Simona D'AguannoAntonietta Moramarco

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